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首页> 外文会议>Water and environment technology conference >The Search for the Habitats of Aquatic Caddisfly Stenopsyche marmorata (Trichoptera) from Environmental DNA using Species-specific Microsatellite Markers
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The Search for the Habitats of Aquatic Caddisfly Stenopsyche marmorata (Trichoptera) from Environmental DNA using Species-specific Microsatellite Markers

机译：使用物种特有的微卫星标记从环境DNA中搜索水生镉蝇（Stennopsyche marmorata）的栖息地

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Environmental DNA (eDNA) is a powerful tool to search the habitats of aquatic animals. The eDNA exists in environmental sample and originate from miscellaneous species inhabiting there. In this study, we employed microsatellite markers to search for the habitats of aquatic caddisfly Stenopysche marmorata from eDNA. The study animal S. marmorata (Trichoptera) is a dominant species in Japan. Microsatellites are short tandem repeat and its marker-primers deigned for single or limited specific species. Therefore, the species-specific PCR using microsatellite markers could amplify the DNA of target animals from the eDNA. We sampled river water and investigated the presence of the S. marmorata in sampling sites. The river water was filtrated by membrane filters (pore size 0.2 μm). The eDNA was extracted by Phenol-Chloroform method and was removed PCR inhibitor. 5 microsatellite markers were employed for species-specific PCR. The sites inhabiting the S. marmorata showed PCR amplification. However, several sites where the S. marmorata was not inhabiting showed also PCR amplification. These sites were located in the downstream of dam's tunnels where S. marmorata inhabited frequently. Flowed eDNA which was produced in upstream might be amplified. These results indicated that the microsatellite markers could work for searing its habitats from eDNA.
机译：环境DNA（eDNA）是搜索水生动物栖息地的强大工具。 eDNA存在于环境样品中，起源于居住在其中的其他物种。在这项研究中，我们使用微卫星标记从eDNA中搜索水生cad虫Stenopysche marmorata的栖息地。在日本，研究动物S. marmorata（Trichoptera）是优势种。微卫星是短串联重复序列，其标记引物可用于单个或有限的特定物种。因此，使用微卫星标记的物种特异性PCR可以从eDNA扩增目标动物的DNA。我们对河水进行了采样，并调查了采样点中沙门氏菌的存在。用膜滤器（孔径0.2μm）过滤河水。通过苯酚-氯仿法提取eDNA，并去除PCR抑制剂。 5种微卫星标记用于物种特异性PCR。栖息于沙门氏菌中的位点显示出PCR扩增。但是，几个不存在沙门氏菌的地方也显示了PCR扩增。这些地点位于沙门氏菌经常居住的水坝隧道的下游。上游产生的流动eDNA可能会被扩增。这些结果表明，微卫星标记可以从eDNA灼伤其栖息地。

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