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首页> 外文会议>Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE >Effect of bicuculline on the spontaneous and evoked activity of patterned embryonic hippocampal neurons cultured in vitro
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Effect of bicuculline on the spontaneous and evoked activity of patterned embryonic hippocampal neurons cultured in vitro

机译：双小分子对体外培养的模式胚胎海马神经元自发和诱发活动的影响

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Characterization of the spontaneous and evoked activity of patterned biological neural networks and discovery of the underlying mechanisms is a major step towards designing functional networks for biosensor, biocomputing and neural prosthetic applications. Here we report on the addition of bicuculline methiodide, a GABAA antagonist, to a low density network of embryonic hippocampal neurons linearly patterned on top of planar microelectrode arrays. Our investigation of the differences in the spontaneous and evoked activities before and after drug addition revealed two distinct behavioral trends. One group of electrodes (Group II) showed a marked increase in spontaneous spike rate, burst rate, and burst duration whereas the other (Group I) was unaffected. Likewise, the reliability of evoked activity was observed to be significantly lower in the presence of bicuculline if the stimulating electrode belonged to Group I as compared with Group II. We discuss how these findings might suggest the existence of cells at various stages of maturity in our culture system.
机译：特征化模式的生物神经网络的自发性和诱发活动的特征以及潜在机制的发现是朝着设计用于生物传感器，生物计算和神经修复应用的功能性网络迈出的重要一步。在这里，我们报道了在平面微电极阵列顶部线性图案化的胚胎海马神经元的低密度网络中，加入了双小分子的甲硫氨酸，一种GABA A 拮抗剂。我们对药物添加之前和之后自发和诱发活动的差异的调查揭示了两种不同的行为趋势。一组电极（II组）显示出自发的尖峰频率，猝发速率和猝发持续时间显着增加，而另一组电极（I组）不受影响。同样地，如果刺激电极属于组I，则与小组II相比，在存在双小分子的情况下，诱发活动的可靠性显着降低。我们讨论了这些发现如何暗示我们培养系统中处于成熟的各个阶段的细胞的存在。

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