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An Integrated Microfluidic System for Early Diagnosis of Breast Cancer in Liquid Biopsy by Using Microrna and FET Biosensors

机译:用MicroRNA和FET生物传感器将液体活组织检查早期乳腺癌早期诊断综合微流体系统

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Breast cancer is one of the leading cancers among women globally with a high mortality rate if not diagnosed in early stages. Liquid biopsy could serve as a non-invasive method for early diagnosis of biomarkers (such as microRNA-195) in blood associated with breast cancer. In this study, we developed an integrated microfluidic system for detecting targeted microRNA-195 by using field-effective-transistor biosensors to quantify an extremely low concentration of microRNA after extraction of extracellular vesicles and microRNAs in plasma. The entire process could be automated with a limit of detection as low as 1 fM. It could be therefore promising for accurate and rapid diagnosis of breast cancer.
机译:如果在早期阶段诊断,乳腺癌是全球女性的主要癌症之一,具有高死亡率。液体活检可以作为与乳腺癌相关的血液中的早期诊断生物标志物(如MicroRNA-195)的非侵入性方法。在这项研究中,我们开发了一种用于通过使用现场有效晶体管生物传感器来检测靶向微团制-195的集成微流体系统,以在血浆中提取细胞外囊泡和微小RORNA后量化极低浓度的微小浓度。整个过程可以通过限制为低至1 fm的检测限。因此,它可能具有准确和快速诊断乳腺癌。

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