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首页> 外文会议>Asia-Pacific conference on environmental electromagnetics >The Gene Expression of Superoxide Dismutase and the changes of the Ultrastructure of Rat Testis After Exposed to Electromagnetics
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The Gene Expression of Superoxide Dismutase and the changes of the Ultrastructure of Rat Testis After Exposed to Electromagnetics

机译：超氧化物歧化酶的基因表达及暴露于电磁测学后大鼠睾丸超微结构的变化

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Aims: To study the expression of superoxide dismutase (CuZnSOD, MnSOD) and the changes of ultrastructure in rat testis after electromagentic radiation(EMR) so as to investigate the mechanisms of EMR affect on procreation. Methods: As an in vivo model, male SD rats were used. Rats were exposured to the field of electromagentics. By reverse transcription-polymerase chain reaction (RT-PCR), manganese SOD (MnSOD) and copper/zinc SOD(CuZnSOD) mRNA in the testis have been tested and the testis samples were prepared for transmission electron microscopy to observe the ultrastructure at 6h, 12h, 24h and 48h after radiation. Results: In rat testis, MnSOD mRNA expression decreased at 24h and CuZnSOD mRNA expression didn't change after radiation, the ratio of MnSOD/CuZnSOD decreased at 24h after radiation. These were some degree injures in the testis at different time after radiation, the testis damage at 24h was serious than that at the other times after radiation. At 24h after exposured, the chromatin was gathered in the border and the endoplasmic reticulum (ER) was dilated and vacuolated in interstitial cell, spermatogonium cell of rat testis. The changes in sustentacular cell showed more serious than the other cells. Conclusion: The results showed that the MnSOD gene expression was decreased and the ultrastructure of rat testis were damaged after exposured to electromagnetics. These changes may induce the other effects, and the change of MnSOD expression might reflect a defensive mechanism of rat body against oxidative stress. If these changes wouldn't regain in time, it may cause the worse effects. Thus, EMR could induce some changes in the testis of rats so as to alter the procreative function.
机译：目的：研究电磁辐射（EMR）后超氧化物歧化酶（CUZNSOD，MNSOD）的表达及大鼠睾丸超微结构的变化，以研究EMR影响对生殖的影响。方法：作为体内模型，使用雄性SD大鼠。大鼠被暴露在电磁学领域。通过逆转录 - 聚合酶链反应（RT-PCR），已经测试了睾丸中的锰SOD（MNSOD）和铜/锌SOD（CUZNSOD）mRNA，并制备睾丸样品用于透射电子显微镜，以观察6小时的超微结构，辐射后12h，24小时和48小时。结果：在大鼠睾丸中，在辐射后24h和Cuznsod mRNA表达的MnSOD mRNA表达没有变化，辐射后24h下的MNSOD / CUZNSOD的比例降低。这些在辐射后不同时间在睾丸中的一定程度损伤，睾丸损伤在24h辐射后的另一次严重。在暴露后24h，将染色质聚集在边界中，在间质细胞，大鼠睾丸的精子细胞中扩张并真空扩张内质网（ER）。缓慢细胞的变化显示比其他细胞更严重。结论：结果表明，MNSOD基因表达降低，大鼠睾丸的超微结构在​​暴露于电磁后损坏。这些变化可能诱导其他效果，并且MNSOD表达的变化可能反映了大鼠体对氧化应激的防御机制。如果这些变化不会及时恢复，可能会导致更糟糕的效果。因此，EMR可以诱导大鼠睾丸的一些变化，以改变生成功能。

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