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Antioxidant activity of wheat extracts as affected by in vitro digestion

机译：体外消化影响的小麦提取物的抗氧化活性

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Phenolic compounds from soft wheat and its milling fractions were extracted and their in vitro antioxidant activity evaluated. Non-hydrolyzed extracts were prepared by extracting phenolics into distilled deionized water. To make hydrolyzed extracts samples were first subjected to pH adjustments in order to simulate gastrointestinal pH conditions. Total phenolic content (TPC) was determined using Folin-Ciocalteu's procedure. Total antioxidant activity (TAA) was determined using Trolox equivalents antioxidant capacity (TEAC) assay and expressed as Trolox equivalents (TE). Antioxidant activity of wheat extracts was determined using the procedures of inhibition of β-carotene bleaching, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and inhibition of oxidation of human low density lipoprotein (LDL) cholesterol. The TPC and TAA of wheat extracts tested were significantly increased following hydrolysis. Similarly, the antioxidant activity, as determined by different proced ures, was considerably increased when the samples were subjected to simulated digestion conditions.
机译：提取来自软小麦的酚类化合物及其研磨级分，并评价其体外抗氧化活性。通过将酚类萃取到蒸馏水中来制备非水解提取物。为了使水解的提取物首先进行pH调节，以模拟胃肠pH值。使用Folin-Ciocalteu的程序确定总酚醛含量（TPC）。使用Trolox当量抗氧化能力（TeaC）测定测定总抗氧化活性（TAA），并表达为拖曳物等同物（TE）。使用β-胡萝卜素漂白的抑制方法测定小麦提取物的抗氧化活性，清除1,1-二苯基-2-富铬（DPPH）的抑制和抑制人低密度脂蛋白（LDL）胆固醇的抑制。在水解后测试的小麦提取物的TPC和TAA显着增加。类似地，当样品进行模拟消化条件时，通过不同的方法ures确定的抗氧化活性显着增加。

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