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Survey of congenital toxoplasmosis by PCR

机译:PCR先天性毒素病变调查

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Introduction: The aim of this study was to determine the incidence of Congenital Toxoplasmosis.Methods: Blood samples of 798 pregnant women were examined by ELISA/IgG and IgM. Acute maternal Toxoplasma gondii infections as well as those with chronic infection were served as case and control groups respectively. Nested PCR and anti T.gondii antibodies by ELISA/IgM were performed on neonates of case and control groups .Data were analyzed by fisher and chi-square tests.Results: five of 798 pregnant women were positive for IgM1/100. Nested PCR showed a 400 bp in three newborn of case group, but was negative in control group (P=0.048). Incident rate of congenital toxoplasmosis was 3.7 per 1000 birth. None of the newborns of case and controls were positive by ELISA.Conclusion: PCR is useful for diagnosis toxoplasmosis in newborn
机译:介绍:本研究的目的是确定先天性毒素症的发生率。通过ELISA / IgG和IgM检查798孕妇的血液样本。 急性母体毒药弓形虫感染以及慢性感染的毒性感染分别作为病例和对照组。 ELISA / IgM的嵌套PCR和抗T.GondII抗体在案例和对照组的新生儿进行,并通过Fisher和Chi-Square Tests分析了Data。结果:798名孕妇中的五个是IGM1 / 100的阳性。 嵌套的PCR在三个新生儿案例组中显示了400磅,但对照组是阴性的(P = 0.048)。 先天性毒素的事件率为每1000次出生3.7。 ELISA的案例和对照的新生儿都没有阳性。结论:PCR可用于新生儿诊断毒素

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