• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Congress of Parasitology >Pantropic retroviral vectors pseudotyped with the glycoprotein of vesicular stomatitis virus (VSVG) mediate gene transfer and transgene expression in Schistosoma japonicum schistosomules
【24h】

Pantropic retroviral vectors pseudotyped with the glycoprotein of vesicular stomatitis virus (VSVG) mediate gene transfer and transgene expression in Schistosoma japonicum schistosomules

机译：腹腔逆转录病毒载体与血清蛋白血栓蛋白（VSVG）介导基因转移和转基因表达在血吸虫血吸虫血吸虫

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Retroviral transduction of cultured schistosomes was explored in our study. The vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse transcriptase gene (hTERT) as a reporter gene under the control of retroviral long terminal repeat (LTR). Pseudotyped virions were employed to transduce Schistosoma japonicum (Sj) to investigate the utility of retrovirus mediated Sj transgenesis and to investigate the activity as human telomerase as a reporter transgene in schistosomes. The presence of transgene hTERT was detected by PCR. Furthermore, analysis of RNAs from transduced parasites, immunohistochemistry of thin sections and immunoblot analysis revealed expression of hTERT transgene in the transduced worms. These findings indicated that Sj could be effectively transduced by VSVG pseudotyped retrovirus carrying the hTERT gene.
机译：我们研究探讨了培养的血吸虫的逆转录病毒转导。 修饰了囊泡口炎病毒糖蛋白（VSVG）-Pseudetyped腹腔逆转录病毒载体pbabe-purO以在逆转录病毒长末端重复（LTR）的控制下将人端粒酶逆转录酶基因（HTERT）掺入报告基因。 使用假型病毒粒子来转移日本血吸虫（SJ）以研究逆转录病毒介导的SJ转基因的效用，并在血吸虫中将活性作为人端粒酶作为报告转基因。 通过PCR检测转基因HTERT的存在。 此外，来自转导寄生虫的RNA，免疫组化和免疫斑分析的分析和免疫斑分析显示出转导蠕虫中HTERT转基因的表达。 这些发现表明SJ可以通过携带HTETT基因的VSVG假型逆转录病毒有效地转导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号