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Genetic variation of glucosinolates in young leaves of winter rapeseed (Brassica napus L.)

机译:冬叶冬叶葡萄糖苷的遗传变异(Brassica Napus L.)

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Increased levels of glucosinolates (GSL) in greenmatter of rapeseed (Brassica napus L.) and other Brassica species are believed to confer resistance to certain pests and diseases. To study the genetic variation of leaf GSL and their genetic correlation with undesired seed GSL, 140 winter rapeseed accessions with a wide range of seed GSL concentrations were grown in the field.Leaf samples were taken in late fall, dried and analyzed by High Performance Liquid Chromatography (HPLC). Seed GSL ranged from 2.1 to 139.5 μmol/g drymatter (DM) ad leaf GSL ranged from 2.4 to 53.7 μmol/g DM with a correlation ofr = 0.58 (p ≤0.01 ). This indicates that there is sufficient genetic variation to select for higher levels of leaf GSL and that to a limited extent this can be done without increasing the undesired seed GSL. Leaf samples were also scanned with Near Infrared Reflectance Spectroscopy (NIRS). A NIRS calibration was developed with the recorded spectra that is suitable to conduct efficient leaf GSL analysis by NIRS for total GSL concetration (1-VR = 0.86) as well as for some individual GSL. For confirmation, a subset of 40 most informative accessions are currently evaluated in field and greenhouse experiments.
机译:被认为是油菜籽(Brassica Napus L.)和其他芸苔属物种的葡糖苷(GSL)水平的水平赋予对某些害虫和疾病的抗性。为研究叶GSL的遗传变异及其与不需要的种子GSL的遗传相关,在田间生长了140个冬季油菜籽,具有各种种子GS1浓度的含量较大。左侧落下,干燥并通过高性能液体干燥并分析色谱(HPLC)。种子GSL从2.1〜139.5μmol/ g帧流/ g叶片GSL范围为2.4至53.7μmol/ g dm,相关性= 0.58(p≤0.01)。这表明在没有增加不期望的种子GSL的情况下,可以在不增加的情况下选择足够的遗传变异来选择更高水平的叶GSL,并且在不增加的情况下,可以在有限的范围内完成。还扫描样品,近红外反射谱(NIRS)扫描。使用录制的光谱开发了NIRS校准,该光谱适于通过NIR进行高效的GSL分析,用于总GSL常数(1-VR = 0.86)以及一些单独的GSL。为了确认,目前在现场和温室实验中评估了40个最具信息性换乘的子集。

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