• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>General Meeting of European Society of Animal Cell Technology >The Production of Human Growth Hormone
【24h】

The Production of Human Growth Hormone

机译：人体生长激素的生产

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

For the production of recombinant proteins for clinical use animal cell cultivation is used. Only these cells are able to perform correct folding and glycosylation of the desired protein. As the production process is expensive and long, cheaper and/orswifter cultivation routines are required. Chinese hamster ovary (CHO) cells were used to examine the expansion of cells and the production of recombinant human growth hormone in different cell culture systems which are supposed to achieve higher cell densities and product concentrations compared to conventional cell culture systems. The CHO cells were grown in suspension in serum-free, low-protein medium. Five different culture systems were used for batch-cultivation: Biostat B, BelloCell 500, spinnerflask, RCCS-D and miniPERM. The systems differed in oxygen supply and medium agitation. While cells are agitated by stirrers in Biostat B and spinner flask, the whole medium is revolved in BelloCell 500, RCCS-D and miniPERM. Unlike the other systems theBelloCell 500 retains the CHO cells on a matrix. The aim was to maximize cell growth and productivity, which was achieved best in BelloCell and RCCS-D. In a second step the influence of temperature on growth and product formation was examined.
机译：对于用于临床使用的重组蛋白，使用动物细胞培养。只有这些细胞能够进行所需蛋白质的正确折叠和糖基化。由于生产过程昂贵，因此需要更长，更便宜，更便宜，更便宜。用于研究不同细胞培养系统中的细胞扩增和重组人生长激素的膨胀，与常规细胞培养系统相比，该不同细胞培养系统中应该实现更高细胞密度和产物浓度的细胞培养系统。 CHO细胞在无血清的低蛋白质培养基中悬浮在悬浮液中生长。五种不同的培养系统用于分批培养：BioStat B，Bellocell 500，Spinnerflash，RCCS-D和MiniPiperm。该系统在氧气供应和中等搅拌中不同。虽然通过搅拌器在BioStat B和Spinner烧瓶中搅拌细胞，但整个培养基在Bellocell 500，RCCS-D和MiniPiPerm中旋转。与其他系统不同，Bellocell 500保留矩阵上的CHO细胞。目的是最大限度地提高细胞生长和生产率，这在Bellocell和RCCS-D中最佳地实现。在第二步中，研究了温度对生长和产物形成的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号