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首页> 外文会议>International Conference on Cytochrome P450 >Isolation of cytochrome P450 genes and their expression in a multiple-herbicide resistant biotype of late watergrass (Echinochloa phyllopogon)
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Isolation of cytochrome P450 genes and their expression in a multiple-herbicide resistant biotype of late watergrass (Echinochloa phyllopogon)

机译：细胞色素P450基因的分离及其在晚期水尔的多除草剂抗性生物型中的表达（Echinochloa phyllogon）

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Incremental herbicide metabolism by cytochrome P450s has been proposed as the basis for multiple-herbicide resistance in late watergrass (Echinochloa phyllopogon). In order to elucidate the mechanism of multiple-herbicide resistance, we isolated P450 genes from late watergrass and compared gene expression patterns between resistant (R) and susceptible (S) biotypes after herbicide treatment. Our results show that expression of CYP71C35, CYP81A12 and CYP89B16 are highly induced in the R biotype, in agreement with the previously reported patterns of P450 activity in microsomal fractions. In addition, CYP71C35 could only be amplified from the genome of an R biotype. The up-regulation patterns and higher expression levels of these genes in the R biotype suggest that these genes could be involved in the herbicide resistance mechanism observed in late watergrass.
机译：通过细胞色素P450S的增量除草剂代谢被提出为晚水石膏中多除草剂抗性的基础（Echinochloa phyllogon）。 为了阐明多除草剂抗性的机制，我们将P450基因分离出晚水的P450基因，并在除草剂处理后进行抗性（R）和易感型生物型之间的基因表达模式。 我们的结果表明，CYP71C35，CYP81A12和CYP89B16的表达在R生物型中高度诱导，与先前报道的微粒体级分中的P450活性模式一致。 此外，CYP71C35只能从R Biotype的基因组扩增。 R Biotype中这些基因的上调模式和更高的表达水平表明这些基因可以参与在晚水道中观察到的除草剂电阻机制。

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