Novel Cysteine-reactive Tandem Mass Tags for Subproteome Labeling, Enrichment and Quantitation

机译：新型半胱氨酸反应性串联标签，用于亚甲基贴标，富集和定量

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Cysteine-reactive Tandem Mass Tags (cysTMT) are novel reagents for targeted labeling of cysteine residues pre- or post-digestion. cysTMT reagents can be used as either isotopic pairs or as an isobaric set for MS-or MS/MS-based multiplexed quantitation. An antibody to the TMT reagent reporter region allows specific detection, capture, and enrichment of labeled proteins and peptides. cysTMT labeling and capture reagents are comparable to ICAT and allow for quantitation of low abundant proteins.

机译：半胱氨酸 - 反应性串联质量标签（Cystmt）是用于靶向标记的半胱氨酸残基预测或消化后的新型试剂。 Cystmt试剂可以用作同位素对或作为MS或MS / MS的多路复用定量的同位素组。 TMT试剂报告区域的抗体允许特异性检测，捕获和富集标记的蛋白质和肽。 Cystmt标记和捕获试剂与Icat相当，允许定量低丰富的蛋白质。

著录项

来源
《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2010年||共1页
会议地点
作者
Michael M. Rosenblatt; Ryan D. Bomgarden; Eric Hommema; Karsten Kuhn; Stefan Kienle; Ian Pike; John C. Rogers;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类 TB994.5-53;
关键词

相似文献

外文文献
中文文献
专利

1. Measurement of S-nitrosylation occupancy in the myocardium with cysteine-reactive tandem mass tags: Short communication [J] . KohrM.J., AponteA., SunJ., Circulation research: a journal of the American Heart Association . 2012,第10期

机译：半胱氨酸反应性串联质量标签测量心肌中S-亚硝基化的占有率：短时通信
2. Sensitive, Efficient Quantitation of 13C-Enriched Nucleic Acids via Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry for Applications in Stable Isotope Probing [J] . Roland Wilhelm, András Szeitz, Tara L. Klassen, Applied and Environmental Microbiology . 2014,第23期

机译：通过高效液相色谱-串联质谱对稳定同位素探测中的应用进行灵敏，高效的13C富集核酸定量
3. Sensitive, Efficient Quantitation of 13C-Enriched Nucleic Acids via Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry for Applications in Stable Isotope Probing [J] . Roland Wilhelm, András Szeitz, Tara L. Klassen, Applied and Environmental Microbiology . 2014,第23期

机译：通过高效液相色谱-串联质谱对稳定同位素探测中的应用进行灵敏，高效的13C富集核酸定量
4. Novel Cysteine-reactive Tandem Mass Tags for Subproteome Labeling, Enrichment and Quantitation [C] . Michael M. Rosenblatt, Ryan D. Bomgarden, Eric Hommema, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：新型半胱氨酸反应性串联质量标签，用于亚甲基签名，富集和定量
5. Development of an assay for fatty acyl-CoAs using liquid chromatography-electrospray ionization-tandem mass spectrometry and its application to stable isotope-labeling and quantitation of sphingolipid metabolism [D] . Haynes, Christopher A. 2009

机译：液相色谱-电喷雾电离串联质谱法测定脂肪酰基辅酶A的研究及其在稳定同位素标记和鞘脂代谢定量中的应用
6. Measurement of S-Nitrosylation Occupancy in the Myocardium With Cysteine-Reactive Tandem Mass Tags [O] . Mark J. Kohr, Angel Aponte, Junhui Sun, -1

机译：用半胱氨酸反应性串联质量标签测量心肌中的S-亚硝基化占用率
7. Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS) [O] . Hediye Erdjument-Bromage, Fang-Ke Huang, Thomas A. Neubert 2018

机译：使用串联质量标签（TMT）和质谱（MS）相对定量蛋白质的样品制剂

Novel Cysteine-reactive Tandem Mass Tags for Subproteome Labeling, Enrichment and Quantitation

摘要

著录项

相似文献

相关主题

期刊订阅