• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics >Characterizing Microbiome Stability and Metabolic Activities in Post-Surgery Crohn's Diseased Human Gut by High Performance Mass Spectrometry
【24h】

Characterizing Microbiome Stability and Metabolic Activities in Post-Surgery Crohn's Diseased Human Gut by High Performance Mass Spectrometry

机译：通过高性能质谱，在手术后克罗恩病人患病人体肠道中表征微生物型稳定性和代谢活动

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The advantages of having "matched" metagenome s for metaproteomes is evident for (i) sampling sequence diversity in a sample and (ii) comparing genes present in the genome with protein actually expressed in a sample. Improved lysis methodology and instrumentation (i.e., LTQ-Orbitrap-Velos-Elite) have provided unprecedented capabilities in the depth and quality of metaproteomic information. This technology provides substantial improvements in sensitivity, speed of mass measurement, and depth of dynamic range for these complex samples. By increasing our ability to identify more peptides from a MS run, better predict peptides with metagenomes, and better predict metagenomic ORFs, we are able to understand what protein functions might be indicative of or influence occurrence of Crohn's Disease. We observed that the gut microbiome environment is fairly stable for Crohn's patients post resection-surgery, but there are some important protein classes that show correlated variations in their response to this environmental perturbation. These results illustrate the power of MS-based proteomics as a strategy for characterizing the stability and metabolic activities of both human and microbial proteins in human gut systems following surgical intervention.
机译：具有“匹配”的Metaprootomes的梅萨克组族的优点是（i）样品中的采样序列分集明显，（ii）比较在样品中实际表达的基因组中存在的基因。改进的裂解方法和仪器（即LTQ-orbitrap-Velos-Elite）在元素组织信息的深度和质量方面提供了前所未有的能力。该技术在这些复杂样品的灵敏度，质量速度和动态范围的深度提供了大量的改进。通过增加我们鉴定来自MS运行的更多肽的能力，更好地预测肽与梅曲线，更好的预测偏见ORF，我们能够理解蛋白质功能可能表明或影响克罗恩病的发生。我们观察到肠道微生物组环境对于切除术后手术的患者相当稳定，但存在一些重要的蛋白质类别，其表现出对这种环境扰动的反应的相关变化。这些结果说明了基于MS的蛋白质组学的力量作为表征手术干预后人体肠系中人体和微生物蛋白的稳定性和代谢活动的策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号