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A NOVEL ENZYMATIC PREPARATION TECHNOLOGY OF NANOCRYSTALLINE CELLULOSE FROM BAMBOO FIBERS

机译:竹纤维纳米晶纤维素的一种新型酶促制备技术

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Nanocrystalline cellulose (NCC), also called cellulose nanocrystals, is typically a rigid rodshaped monocrystalline cellulose domain (whisker) with 1-100 nm in diameter and tens to hundreds of nanometers in length. Traditionally, NCC is almost prepared by acid hydrolysis and mechanical treatment, and the raw materials mainly focus on wood cellulose, microcrystalline cellulose (MCC) and animal fibres. In this contribution, enzymatic hydrolysis was chosen to produce cellulose nanocrystals. After the homogenizer, a kind of commercial cellulase, viz. enzyme Cclast (endoglucanase) was used in the process of enzymatic hydrolysis. The effect of enzymatic treatment on the morphology of the ensuing particles is reported. The prepared cellulose nanocrystals were morphologically characterized by transmission electron microscopy (TEM) and the sugar analyses of hydrolysis residues were characterized by ionic chromatography. The degree of polymerization (DP) was characterized by automatic viscosimeter. All bamboo nanofibers presented a rod-like shape, an average diameter (D) of 5.3 nm and length (L) of 221 nm, with an aspect ratio (L/D) of around 42. The use of endoglucanases allows obtaining a mixture of microfibrillated cellulose (MFC) and rod-like nanoparticles.
机译:纳米晶纤维素(NCC),也称为纤维素纳米晶体,通常是刚性杆状单晶纤维素结构域(晶须,直径为1-100nm,长度为数百纳米。传统上,NCC几乎通过酸性水解和机械处理制备,原料主要关注木材纤维素,微晶纤维素(MCC)和动物纤维。在该贡献中,选择酶水解以产生纤维素纳米晶体。在均化器之后,一种商用纤维素酶,VIZ。在酶水解过程中使用酶CCLAST(内切葡聚糖酶)。据报道了酶处理对随后颗粒形态的影响。通过透射电子显微镜(TEM),制备的纤维素纳米晶体形态学表征,并且通过离子色谱法表征了水解残留物的糖分析。通过自动吸附计表征聚合度(DP)。所有竹纳米纤维呈棒状形状,平均直径(d)为5.3nm,长度(l)为221nm,纵横比(l / d)约为42.使用内切葡聚糖酶允许获得混合物微纤化纤维素(MFC)和棒状纳米颗粒。

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