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Polarized Raman investigations of oriented animal muscle fibers affected by storage time applying a 671 nm diode laser

机译：使用671 nm二极管激光器对受存储时间影响的定向动物肌肉纤维进行的偏振拉曼研究

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Due to its analytical ability and sensitivity to molecular vibrations, Raman spectroscopy provides valuable information of the secondary structure of proteins. Moreover, polarized Raman spectroscopy is shown to be a useful instrument to investigate the structural changes resulting from the aging and spoilage process of meat. In this work, polarized Raman spectra were measured on oriented cuts of pork and turkey. Fresh meat slices were stored at 5 °C and measured for a consecutive time period of 10 days. A 671 nm microsystem diode laser was used as excitation light source. The laser power at the sample was 50 mW and the integration time of each Raman spectrum was set to 5 seconds. Measurements were performed with a laser beam orientation perpendicular to the long axis of the muscle fibers. In that arrangement, the fibers were aligned either parallel or perpendicular to the polarization direction of the laser source. By using the statistical method of principal components analysis (PCA), a clear separation of the meat samples can be found for fresh meat according to the orientation (parallel or perpendicular) using the first two principal components. During the storage period, this separation subsequently vanishes due to the aging process and due to an increase of the microbial spoilage of the meat surface. For the latter effect, a time-dependent distinction of the Raman spectra is presented as well. Furthermore, specific changes of conformation-sensitive Raman bands were recognized, notably a decrease of the intensities of α-helical protein conformation.
机译：由于其分析能力和对分子振动的敏感性，拉曼光谱学提供了蛋白质二级结构的宝贵信息。此外，偏振拉曼光谱被证明是研究由肉的老化和变质过程引起的结构变化的有用工具。在这项工作中，在猪肉和火鸡的定向切片上测量了偏振拉曼光谱。将新鲜的肉切片保存在5°C，连续10天进行测量。 671 nm微系统二极管激光器用作激发光源。样品处的激光功率为50 mW，每个拉曼光谱的积分时间设置为5秒。用垂直于肌纤维长轴的激光束定向进行测量。在这种布置中，光纤平行于或垂直于激光源的偏振方向排列。通过使用主成分分析（PCA）的统计方法，可以使用前两个主成分根据方向（平行或垂直）对新鲜肉类的肉样品进行清晰分离。在储存期间，由于老化过程和肉表面微生物变质的增加，这种分离随后消失。对于后一种效应，还提出了拉曼光谱的时间依赖性。此外，认识到构象敏感性拉曼带的特定变化，特别是α-螺旋蛋白构象强度的降低。

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