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Isolation and Purification of Polysaccharides from Pholiota adiposa

机译：来自Pholiota Adiposa多糖的分离和纯化

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Pholiota adiposa is an edible mushroom with excellent nutritional and medicinal properties. After culture in fermentation medium, P. adiposa mycelia were filtered, lyophilized and powdered. A crude polysaccharide (PAP) of P. adiposa was prepared from the mycelial powder with hot water, centrifuged and the resulting supernatant lyophilized. PAP was fractionated by 30%, 60% and 80% ethanol precipitation steps to yield PAP30, PAP60 and PAP80. Subsequently PAP30-1 and PAP30-2, PAP60-1 and PAP602, and PAP80-1 and PAP80-2 were isolated from PAP30, PAP60 and PAP80, respectively, by ion-exchange chromatography on a DEAE-Sepharose column. Polysaccharide content increased from 43. 8% in PAP to 50. 54%-73. 19% in PAP30-1 ~ PAP80-2. The protein content was 4. 92% at minimum in these polysaccharide products. In order to identify the chemical composition, the six polysaccharides (PAP30-1, PAP302, PAP60-1, PAP60-2, PAP80-1 and PAP80-2) were further purified by gel-filtration on Sephacrvl S-100 -500. Finally, three water-soluble polysaccharides (PAP30-2a, PAP60-2b and PAP80-2a) were obtained. HPLC analysis revealed that PAP30-2a, PAP60-2b and PAP802a exhibited a molecular weight of 6. 6×105Da, 8.4× 103Da and 3. 5×103Da, respectively.
机译：Pholiota Adiposa是一种食用蘑菇，具有优异的营养和药用特性。在发酵培养基中培养后，将P.Adiposa菌丝体过滤，冻干和粉状。用热水，离心和所得上清液从菌丝粉末制备P.Adiposa的粗多糖（PAP）。 PAP分馏30％，60％和80％乙醇沉淀步骤，得到PAP30，PAP60和PAP80。随后PAP30-1和PAP30-2，PAP60-1和PAP602，PAP60-1和PAP80-1和PAP80-2分别通过DEAE-Sepharose柱上的离子交换色谱法分离PAP30，PAP60和PAP80。多糖含量从PAP的43％增加到50.54％-73。 PAP30-1 ~PAP80-2中的19％。在这些多糖产物中，蛋白质含量最小为4. 92％。为了鉴定化学成分，通过凝胶过滤在Sephacrvl S-100 -500上进一步纯化六种多糖（PAP30-1，PAP302，PAP60-1，PAP60-2，PAP80-1和PAP80-2）。最后，获得了三种水溶性多糖（PAP30-2A，PAP60-2B和PAP80-2A）。 HPLC分析显示，PAP30-2A，PAP60-2B和PAP802A分别显示出6.6×105DA，8.4×103DA和3.5×103DA的分子量。

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