DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer

机译：使用用金层改性的可光可寻性电位传感器进行DNA杂交检测

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Single-stranded deoxyribonucleic acid (ssDNA) probes modified with thiol group were immobilized on the patterned gold surface of a light-addressable potentiometric sensor (LAPS) via gold-thiol bond. The LAPS chip immobilized with ssDNA probe was used as a working electrode in a scanning LAPS measurement system. After the working electrode was incubated with a solution containing strands of complementary target ssDNA, the photocurrent-voltage curves of LAPS shift with increasing target ssDNA concentration. On the contrary, in a control experiment, practically no shift of photocurrent-voltage curve was observed if the LAPS chip was incubated with a solution containing mismatch ssDNA. This provides a convenient and label-free method for the detection of DNA hybridization and therefore, a promising approach for the development of novel LAPS-based DNA chips.

机译：用硫醇组改性的单链脱氧核糖核酸（SSDNA）探针通过金 - 硫醇键固定在可光寻常型电位传感器（圈）的图案化金表面上。用SSDNA探针固定的圈芯片用作扫描圈测量系统中的工作电极。将工作电极与含有互补靶SSDNA的括号的溶液一起温育之后，随着靶SSDNA浓度的增加，圈的光电流曲线。相反，在对照实验中，如果用含有错配SsDNA的溶液温育液体芯片，则几乎不会观察到光电流曲线的偏移。这为检测DNA杂交的检测提供了一种方便和无标记的方法，因此是一种有前途的基于圈的DNA芯片的开发方法。

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《ITG/GMA Symposium on Sensors and Measuring Systems》|2014年||共4页
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Wu Chunsheng; Bronder Thomas; Poghossian Arshak; Werner Carl Frederik; Schoening Michael J.;
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DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer

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