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首页> 外文会议>The Meeting of the International Humic Substances Society >pH Dependence of Configurations and Surface Properties of Microbial Extracellular Polymeric Substances (EPS)
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pH Dependence of Configurations and Surface Properties of Microbial Extracellular Polymeric Substances (EPS)

机译：微生物细胞外聚合物物质（EPS）构造和表面性质的pH依赖性（EPS）

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The flocculability of microorganisms plays a crucial role in bioreactors and is substantially affected by pH. However, the mechanism for such an effect remains unclear. In this work, the pH dependence of configuration and surface property of microbial extracellular polymeric substances (EPS), excreted from Bacillus megaterium TF_(10), and accordingly its flocculability is elucidated. The main functional groups and buffering zones in the EPS were identified from FTTR and acid-base titration. The laser light scattering analysis revealed that the deprotonated or protonated states of these functional groups in EPS were governed by pH and resulted in more dense and compact structure at a lower pH because of hydrophobicity and intermolecular hydrogen bonds. The zeta potential measurements implied that the isoelectric point and the electrostatic repulsion action of EPS were controlled by pH. These results clearly demonstrate that the EPS configurations and accordingly microbial flocculability are heavily dependent on pH in solution.
机译：微生物的絮凝起着在生物反应器的关键作用，并基本上由pH值的影响。然而，对于这样的效果的机制仍不清楚。在这项工作中，配置和微生物细胞外聚合物质（EPS），巨大芽孢杆菌TF_（10）排出的表面特性，并且相应地其絮凝的pH依赖性阐明。在EPS的主要官能团和缓冲区域从FTTR和酸 - 碱滴定进行鉴定。激光散射分析表明，在EPS这些官能团的去质子化或质子化状态通过在pH值由于疏水性和分子间氢键较低pH支配并导致更致密的和结构紧​​凑。 ζ电位测量暗示，等电点和EPS的静电排斥作用，通过在控制pH。这些结果清楚地表明，EPS配置，并相应地微生物絮凝严重依赖于pH值在溶液中。

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