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首页> 外文会议>International Conference on Solid-State Sensors, Actuators and Microsystems amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Eurosensors >A Bubble-Free and Low-Shear-Stress Microfluidic Device for High-Quality Monitoring of Zebrafish Embryonic Development
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A Bubble-Free and Low-Shear-Stress Microfluidic Device for High-Quality Monitoring of Zebrafish Embryonic Development

机译：一种无泡沫和低剪切应力的微流体装置，用于斑马鱼胚胎发育的高质量监测

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摘要

This paper presents a microfluidic device for the capture and culture of zebrafish embryos and real-time monitoring of early-stage embryonic development. Innovative claims include: (i) rapid-prototyping and low-cost fabrication and easy-to-operate device; (ii) the use of a degassing chamber to remove air bubbles during live imaging; and (iii) the optimal embryo-trapping regime to reduce potential shear stress on zebrafish embryos. Long-term monitoring of zebrafish embryogenesis at early stages verifies that the microfluidic device enables the reliable capture and culturing of zebrafish embryos under continuous medium perfusion and high-quality screening of their developmental dynamics.
机译：本文介绍了一种微流体装置，用于斑马鱼胚胎的捕获和培养和早期胚胎发育的实时监测。创新索赔包括：（i）快速原型和低成本的制造和易于操作的设备; （ii）使用脱气室在实时成像期间除去气泡; （iii）最佳的胚胎捕获制度，以减少斑马鱼胚胎上的潜在剪切应力。早期阶段的斑马鱼胚胎发生的长期监测验证了微流体装置在连续中灌注和高质量筛选下，微流体装置能够可靠地捕获和培养斑马鱼胚胎的发育动力学。

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