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Nanomechanical Properties of Dentin Treated with a CO_2 Laser for Potential Caries Inhibition

机译:用CO_2激光治疗牙本质的纳米机械性能,以进行潜在龋抑制作用

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The effect of pulsed CO_2-laser treatments on the microstructure and nanomechanical properties was studied using atomic force microscopy and nanoindentation. The microstructure and roughness of dentin specimens treated by a 9.3 μm CO_2 laser at fluences of 0.5; 0.75; 1.0; and 1.5 J/cm~2 were evaluated and the elastic modulus and hardness before and after a nine days demin/remin application for laser treatments at 0.75 and 1.5 J/cm~2 were determined. The hypothesis that CO_2-laser-treatments reduce or inhibit the demineralization process was tested. Surface cracks were observed at fluences of 0.75 J/cm~2 and higher. Surface roughness increased strongly after laser irradiation and was about 10 times higher at maximum fluence. Mechanical properties profiles from the laser treated surface to sound dentin showed increased modulus and hardness values at dentin surfaces treated, indicating removal of organic phases and reinforcement of the tissue by increased mineral content. Mechanical properties decreased after demin/remin application in all groups and the hardened surface layer disappeared for samples treated at 0.75 J/cm~2. While increased properties were still observed in the laser affected surface zone for treatments at 1.5 J/cm~2, demineralization was severe underneath these layers as indicated by a sharp drop in properties. The depth of demineralization was slightly decreased in the laser-treated group compared to the untreated controls. We conclude that CO_2-laser treatments produce dentin surfaces with increased nanomechanical properties. These surface layers do not, however, provide protection against demineralization due to surface cracking and possible delamination. Moreover, the surface cracks induced by the laser-irradiation raise concerns about a clinical potential for caries inhibition in dentin.
机译:采用原子力显微镜和纳米狭窄研究了脉冲CO_2激光处理对微观结构和纳米力学性质的影响。用9.3μmCO_2激光处理牙本质标本的微观结构和粗糙度在0.5的流量下。 0.75; 1.0;测定了1.5J / cm〜2,测定了9天Demin / Remin施用在0.75和1.5J / cm〜2的激光处理前后的弹性模量和硬度。测试了CO_2激光治疗减少或抑制脱矿化过程的假设。在流量为0.75J / cm〜2和更高的情况下观察到表面裂缝。激光照射后表面粗糙度强烈升高,最大流量较高约10倍。从激光处理表面到声牙的机械性能曲线显示在处理的牙本质表面上的模量和硬度值增加,表明通过增加的矿物质含量去除有机相和增强组织。在所有基团中的Demin / Remin施用中施加后的机械性能降低,硬化表面层消失在0.75J / cm〜2的样品中。虽然在激光受影响的表面区中仍观察到增加的性能,但在1.5J / cm〜2的处理中,脱矿质在这些层下方严重,如急剧下降所示。与未处理的对照相比,激光处理组中的脱矿质深度略微降低。我们得出结论,CO_2激光治疗具有纳米机械性能增加的牙本质表面。然而,这些表面层不会提供由于表面裂化和可能的分层而导致的脱矿质的保护。此外,激光辐照诱导的表面裂缝引起了牙本质肽抑制的临床潜力的担忧。

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