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Bioconjugates of luminescent CdSe-ZnS quantum dots with engineered recombinant proteins: novel self-assembled tools for biosensing

机译：具有工程重组蛋白的发光Cdse-Zns量子点的生物缀合物：新型自组装工具用于生物传感

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Colloidal semiconductor quantum dots (QDs) are luminescent nanoparticles with size-dependent emission spectra spanning a wide range of wavelengths in the visible and near IR. This property, as well as their higher resistance to photo-degradation compared to organic dye labels, makes QDs potentially suitable for certain biomolecule tagging and multiplexing applications. We describe an electrostatic self-assembly approach for conjugating highly luminescent colloidal CdSe-ZnS core-shell QDs with engineered two-domain recombinant proteins to forrn conjugates for sensing and imaging applications. The design, preparation, and characterization of high quantum yield IgG antibody-binding protein G bioconjugates using luminescence, electrophoretic gel shift, and affinity assays is reported.

机译：胶体半导体量子点（QDS）是具有尺寸依赖性发射光谱的发光纳米颗粒，该发射光谱跨越可见和接近IR的各种波长。与有机染料标签相比，这种特性以及它们对光降解的更高抗性，使得QD可能适用于某些生物分子标记和复用应用。我们描述了一种静电自组装方法，用于将高发光胶体Cdse-Zns核QDS与工程化的双结构域重组蛋白缀合，以进行用于感测和成像应用的缀合物。报道了使用发光，电泳凝胶偏移和亲和力测定的高量子产率IgG抗体结合蛋白G生物缀合物的设计，制备和表征。

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《Symposium on Semiconductor Quantum Dots》|2001年||共6页
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Ellen R. Goldman; Hedi Mattoussi; Phan T. Tran; George P. Anderson; J. Matthew Mauro;
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中图分类半导体量子理论;
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1. Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins [J] . Goldman ER, Medintz IL, Hayhurst A, Analytica chimica acta . 2005,第1期

机译：使用工程化的多组氨酸末端蛋白制备的自组装发光CdSe-ZnS量子点生物缀合物
2. Bioconjugation of highly luminescent colloidal CdSe-ZnS quantum dots with an engineered two-domain recombinant protein [J] . Mattoussi H., Goldman ER., Green TM., Physica status solidi, B. Basic research . 2001,第1期

机译：工程双域重组蛋白与高发光胶体CdSe-ZnS量子点的生物缀合
3. Use of luminescent CdSe-ZnS nanocrystal bioconjugates in quantum dot-based nanosensors [J] . Tran PT., Goldman ER., Anderson GP., Physica status solidi, B. Basic research . 2002,第1期

机译：发光CdSe-ZnS纳米晶体生物共轭物在基于量子点的纳米传感器中的应用
4. Bioconjugates of luminescent CdSe-ZnS quantum dots with engineered recombinant proteins: Novel self-assembled tools for biosensing [C] . J. Matthew Mauro, George P. Anderson, Phan T. Tran, Symposium on Semiconductor Quantum Dots . 2001

机译：具有工程重组蛋白的发光Cdse-Zns量子点的生物缀合物：新型自组装工具用于生物传感
5. Luminescent quantum dot and protein composite nanoparticles for bioanalytical applications. [D] . Wicks, Arriel. 2010

机译：用于生物分析的发光量子点和蛋白质复合纳米粒子。
6. Self-Assembled Quantum Dot-Peptide Bioconjugates for Selective Intracellular Delivery [O] . James B. Delehanty, Igor L. Medintz, Thomas Pons, -1

机译：自组装的量子点肽生物共轭物的选择性细胞内递送。
7. Preparation and Third-Order Optical Nonlinearity of Self-Assembled Chitosan/CdSe-ZnS Core-Shell Quantum Dots Multilayer Films [O] . Xincheng Shen, Daiwen Pang 2014

机译：自组装壳聚糖/ Cdse-Zns核壳量子点多层膜的制备及三阶光学非线性
8. Use of Luminescent CdSe-ZnS Nanocrystal Bioconjugates in Quantum Dot- Based Nanosensors [R] . Tran, P. T. , Goldman, E. R. , Anderson, G. P. , 2002

机译：在量子点基纳米传感器中使用发光Cdse-Zns纳米晶体生物共轭物

Bioconjugates of luminescent CdSe-ZnS quantum dots with engineered recombinant proteins: novel self-assembled tools for biosensing

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