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首页> 外文会议>International Symposium on Ganoderma Research >Effect of Green Valley Lingzhi on Monocyte Adhesion to Endothelium and Adhesion Molecule Expression
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Effect of Green Valley Lingzhi on Monocyte Adhesion to Endothelium and Adhesion Molecule Expression

机译：绿谷灵芝对内皮和粘附分子表达单核细胞粘附的影响

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This research studied the effect of sera derived from rats administrated Green Valley Lingzhi (GVL) on low- density lipoprotein (LDL) oxidation, monocyte adhesion to the human endothelial cells line ECV304 (EC) and the expression of adhesion molecules induced by oxidized-LDL (ox-LDL) and glycated albumin. LDL oxidation was determined by measuring the TBA- reactive substances in the supernatants expressed as malondialdehyde equivalents (nmol/100μL medium). Monocyte adhesion to the EC was determined by measuring myeloperoxidase activity of adherent monocyte. The expression of ICAM-1 and VCAM-1 was determined by cell ELISA and flow cytometric technique. The sera derived from the rats administrated 0.12g/kg of GVL once (0.5h, 1h, 2h, 3h after administration) or two times (0.5h, 1h after administration) were not exhibited significant effects on LDL oxidation, but the sera from the rats administrated 0.12g/kg (2h and 3h after administration) or 0.12g/kg, 0.24g/kg and 0.72g/kg GVL sera (p. o for 10 days) showed the significant effects on reducing LDL oxidation (P<0.05); GVL sera (0.12g/kg, 0.24g/kg and 0.72g/kg GL for 10 days) inhibited monocyte adhesion to EC induced by AGE(P<0.05), and 0.24g/kg and 0.72g/kg inhibited monocyte adhesion to EC induced by ox-LDL(P<0.05). The results by cell ELISA method showed that the GL sera (0.24g/kg, 0.72g/kg and 0.72g/kg for 10 days) produced significant effects of inhibiting the expression of ICAM-1 induced by ox-LDL and expression of ICAM-1 and VCAM-1 induced by glycated albumin. GVL decreased LDL oxidation, inhibited monocyte adhesion to the EC by decreasing the expression of adhesion molecules.
机译：本研究研究了大鼠的血清的效果上低密度脂蛋白（LDL）氧化，单核细胞粘附到人内皮细胞系ECV304（EC）和粘附分子的由氧化LDL诱导的表达给予绿谷灵芝（GVL） （OX-LDL）和糖化白蛋白。 LDL氧化通过测量表示为丙二醛等价物（纳摩尔/100μL培养基）的上清中的TBA-反应物质来确定。单核细胞粘附至EC通过测量粘附的单核细胞的髓过氧化物酶活性来确定。 ICAM-1和VCAM-1的表达通过细胞ELISA测定和流式细胞术技术。从大鼠衍生的血清给药0.12克/ kg的GVL的一次（0.5H，1H，2H，给药后3小时）或两次（0.5H，给药后1小时）均不能发挥对LDL氧化显著效果，但是从血清大鼠给药0.12克/公斤（2H和给药后3小时）或0.12克/千克，0.24克/ kg和0.72克/ kg的GVL血清（第o表示10天）显示出对减少LDL氧化（p的显著效果< 0.05）; GVL血清（0.12克/千克，0.24克/ kg和0.72克/ kg的GL为10天）抑制单核细胞粘附至EC由AGE诱导（P <0.05），和0.24克/ kg和0.72克/ kg的抑制单核细胞粘附到EC诱导氧化低密度脂蛋白（P <0.05）。通过细胞ELISA法的结果表明，GL血清（0.24克/千克，0.72克/ kg和0.72克/ kg的10天）产生抑制的表达的效果显著ICAM-1通过的ox-LDL和ICAM的表达诱导-1和VCAM-1诱导的糖化白蛋白。 GVL通过降低粘附分子的表达LDL氧化作用，抑制单核细胞粘附降低到EC。

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