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首页> 外文会议>International Symposium on Ganoderma Research >The Antitumor and Anti-angiogenic Activity of Ganoderma lucidum Polysaccharides
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The Antitumor and Anti-angiogenic Activity of Ganoderma lucidum Polysaccharides

机译：灵芝多糖的抗肿瘤和抗血管生成活性

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Ganoderma lucidum has been widely used as a medicine to promote health and longevity in China for thousands of years. A series of experiments have demonstrated that the antitumor activity of Ganoderma lucidum polysaccharides (Gl-PS) may be mediated to some extent by activation of host immune functions. In this study, we investigate the anti-angiogenic activity of Ganoderma lucidum polysaccharide (Gl-PS) on tumor growth, which maybe a new pathway of antitumor effect by Gl-PS. In present study, we investigated the antitumor activity of Gl-PS. BALB/c mice which implanted with Sarcoma 180 were administrated intragastrically with Gl-PS 50, 100, 200mg·kg<'-1> respectively for 10 days. At the end of experiment, the tumor were cut and weighted. The effect of Gl-PS and Gl-PS-treated serum on proliferation of human lung carcinoma cell line PG were detected by MTT assay; BALB/c nude mice were implanted with xenograft (human lung carcinoma cell line PG). Gl-PS were given at the dose of 50, 100, 200mg·kg<'-1> intragastrically for 32 days, then the tumor were cut and weighted. At the same time, spleen lymphocytes of the nude mice were also prepared to observe the effect of GL-PS on lymphocytes proliferation induced by 2.5μg·ml<'-1> ConA or 5μg·ml<'-1> LPS. The influence of Gl-PS on neutral red phagocytosis of nude mice peritoneal macrophages were also investigated in vitro. Gl-PS 40, 80μg·ml<'- 1> and Gl-PS-treated serum 10μl were added to the CAM (chick embryo chorioallantoic membrane) respectively to investigate the anti-angiogenesis effect of Gl-PS. The result showed that Gl-PS inhibited the growth of Sarcoma 180 significantly which had been implanted in BALB/c mice in vivo. Gl-PS inhibited the growth of Sarcoma 180 in a dose-dependent manner. The inhibitory rates were 35.20%, 45.25%, 61.88%, respectively. Gl-PS directly adding to the cultured medium did not inhibit PG cell proliferation in vitro, but 50, 100, 200mg·kg<'-1> Gl- PS-treated serum significantly inhibited PG cell proliferation, the inhibitory ratios were 22.54%, 26.76%, 30.28%. In a further study, Gl-PS 50, 100, 200mg·kg<'-1> can also inhibit the xenograft (human lung carcinoma cell line PG) in BALB/c nude mice significantly in vivo and the inhibitory rates were 55.47%, 46.04%, 46.79%, respectively. At the same time, lymphocyte proliferation experiment indicated that nude mice lymphocytes could be stimulated by LPS but not by ConA. The results above demonstrated that the cell mediated immunity was default, but Gl- PS still has high anti-tumor activity, which indicating that GL might be have other anti-tumor paths besides cell mediated immunity. The CAM assay showed that Gl-PS treated serum had anti- angiogenesis effect. In conclusion, the anti-angiogenesis of tumor may be a new mechanism of Gl-PS on tumor inhibition.
机译：Ganoderma Lucidum已被广泛用作促进中国促进千年健康和长寿的药物。一系列实验表明，可以通过激活宿主免疫功能来介导Ganoderma lucidum多糖（GL-PS）的抗肿瘤活性。在这项研究中，我们研究了灵芝多糖（GL-PS）对肿瘤生长的抗血管生成活性，这可能是GL-PS的抗肿瘤效果的新途径。在目前的研究中，我们研究了GL-PS的抗肿瘤活性。植入肉瘤180的BALB / C小鼠分别用GL-PS 50,100,200mg·kg <-1>造成胃内造成胃内施用10天。在实验结束时，切割并加权肿瘤。通过MTT测定检测GL-PS和GL-PS处理血清对人肺癌细胞系PG的增殖的影响;将BALB / C裸鼠植入异种移植物（人肺癌细胞系PG）。 GL-PS以50,100,200mg·Kg < - 1>胃肠的剂量给出32天，然后切割并加权肿瘤。同时，还制备裸鼠的脾脏淋巴细胞，以观察到GL-PS对由2.5μg·ml -1> Cona或5μg·ml -1> LPS诱导的淋巴细胞增殖的影响。在体外还研究了GL-PS对裸鼠腹膜巨噬细胞中性红吞噬作用的影响。将GL-PS40,80μg·mL -1>和GL-PS处理的血清10μl加入凸轮（鸡胚型肠膜膜）中，研究GL-PS的抗血管生成效果。结果表明，GL-PS显着抑制了肉瘤180的生长，其在体内植入Balb / C小鼠中植入。 GL-PS以剂量依赖的方式抑制SARCOMA 180的生长。抑制率分别为35.20％，45.25％，61.88％。 GL-PS直接加入培养介质的体外不抑制PG细胞增殖，但50,100,200mg·Kg < - 1> GL-PS治疗的血清显着抑制PG细胞增殖，抑制比率为22.54％， 26.76％，30.28％。在进一步的研究中，GL-PS 50,100,200mg·Kg < - 1>也可以在体内显着抑制Balb / C裸鼠中的异种移植物（人肺癌细胞系PG），抑制率为55.47％，分别为46.04％，46.79％。同时，淋巴细胞增殖实验表明裸鼠淋巴细胞可以通过LPS刺激但不是由CONA刺激。以上结果证明了细胞介导的免疫默认，但GL-PS仍然具有高的抗肿瘤活性，这表明除细胞介导的免疫之外，目录可能具有其他抗肿瘤途径。凸轮测定显示GL-PS处理的血清具有抗血管生成效应。总之，肿瘤的抗血管生成可能是GL-PS对肿瘤抑制的新机制。

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