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首页> 外文会议>International Symposium on Ganoderma Research >Ganoderma lucidum Inhibits PC-3 and HUVEC Cells in vitro
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Ganoderma lucidum Inhibits PC-3 and HUVEC Cells in vitro

机译：Ganoderma lucidum在体外抑制PC-3和Huvec细胞

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Cancer is one of the leading causes of death in the world despite newly developed tools for diagnosis and treatment. Much effort has been made in the search for dietary supplements as cancer chemopreventive and chemotherapeutic agents. Ganoderma lucidum has been used in herbal medicine in China for more than 2000 years. Numerous reports have been published on health promoting beneficial effects of G. lucidum. However, most studies have been focused on water-soluble components of the fruiting body of G. lucidum. In this study we examined the effect of G. lucidum alcoholic extracts from both spore and fruiting bodies on the inhibition of prostate and breast cancer cell growth and angiogenesis. G. lucidum spore was first extracted with ethanol and water. Strategy of in vitro bioassay guided fractionation was employed. The active ethanol extract was then partitioned between hexane, ethyl acetate and methanol. Human breast cancer cells (MDA-MB231) and human prostate cancer cells (PC-3) were employed to evaluate anti-proliferative effects of each extract. Human umbilical vein endothelial cells (HUVECs) was used to test the anti-angiogenic effect. MDA-MB231 and HUVECs cell number after 48h incubation and PC-3 cell number after 72h incubation were quantitated using <'3>H-thymidine incorperation. Ethyl acetate fraction of spore reduced PC-3 cell growth by 39% and 66% at 80 and 250μg/ml, and reduced HUVEC cell growth by 61% and 80% at 20 and 40μg/ml respectively. Study of fruiting bodies revealed ethanol extract inhibited PC-3 cell growth in a dose responsive manner in the concentration less than 250μg/ml. However, neither fruiting bodies nor spore ethanol extract inhibited the growth of MDA-MB231 cells. In conclusion we found ethanol extract from both G. lucidum spore and fruiting bodies showed significant anti-angiogenic activities. Furthermore, this extract showed inhibitory effect on prostate cancer cell growth but not breast cancer cell. Further study will include the identification of active components from the spore and fruiting bodies and examining the effect of active components on in vitro and in vivo breast cancer proliferation and angiogenesis.
机译：尽管新开发的诊断和治疗工具，癌症是世界上的主要死因之一。在寻找膳食补充剂作为癌症化学预防和化学治疗剂的膳食补充剂中取得了很多努力。 Ganoderma lucidum已在中国的草药中使用超过2000年。已发表众多报告关于促进G. lucidum的有益效果。然而，大多数研究已经集中在G. lucidum的结果体内的水溶性组分上。在这项研究中，我们研究了G. lucidum酒精提取物从孢子和乳腺癌的效果对前列腺和乳腺癌细胞生长和血管生成的抑制作用。 G. Lucidum孢子首先用乙醇和水提取。使用体外生物测定的策略引导分馏。然后将活性乙醇提取物在己烷，乙酸乙酯和甲醇之间分配。使用人乳腺癌细胞（MDA-MB231）和人前列腺癌细胞（PC-3）来评估每种提取物的抗增殖效果。人脐静脉内皮细胞（HUVECS）用于测试抗血管生成效果。使用<'3> H-Trymidine Buporatal定量，在48h孵育和PC-3细胞数之后，MDA-MB231和HUVECS细胞数。孢子的乙酸乙酯馏分降低了30％和250μg/ mL的PC-3细胞生长39％和66％，分别将HUVEC细胞生长减少61％和80％，分别为20和40μg/ mL。结果研究结果显示乙醇提取物在浓度小于250μg/ mL的浓度下以剂量响应方式抑制PC-3细胞生长。然而，既不是结果体也不是孢子乙醇提取物抑制MDA-MB231细胞的生长。总之，我们发现来自G. lucidum孢子和结果体的乙醇提取物显示出显着的抗血管生成活性。此外，该提取物对前列腺癌细胞生长而不是乳腺癌细胞显示出抑制作用。进一步的研究将包括从孢子和结果体中鉴定活性组分，并检查活性组分对体外和体内乳腺癌增殖和血管生成的影响。

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