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首页> 外文会议>International Symposium on Ganoderma Research >Purification and Identification of Glycopeptides from Ganoderma lucidum Fruit Bodies Cultivated with Grass and Wood Log
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Purification and Identification of Glycopeptides from Ganoderma lucidum Fruit Bodies Cultivated with Grass and Wood Log

机译：用草和木材栽培的灵芝果实糖肽的纯化和鉴定

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摘要

This research studied the amount and properties of glycopeptides of Ganoderma lucidum cultivated with wood log (GLPW) and grass (GLPG), and investigated the feasibility of substitution of grass for wood log in cultivate Ganoderma lucidum. Glycopeptides were extracted and isolated from fruit bodies of Ganoderma lucidum followed by dialysis. The component sugar and molecular weight distribution of the glycopeptides were determined by GC and HPLC. The structure of the glycopeptides was detected by IR, <'1>H NMR and <'13> C NMR. The molecular weight of the glycopeptides of GLPW and GLPG was Mw 5.13×10<'5> and 5.85×10<'5>,Mη 6.21× 10<'5> and 6.96×10<'5> respectively. The glycopeptides from both GLPW and GLPG were linked mainly through β-glycosidic bond and consisted of 16 amino acids as well as Rha, Xyl, Fru, Gal and Glc (There was small amount of Man in GLPW). The physical properties and chemical components of GLPW and GLPG were very similar (or have minimal differences). The glycopeptide extracting rate of GLPG was 2.8 times to GLPW.
机译：这项研究研究了量和木材原木（GLPW）和草（GLPG）栽培灵芝糖肽的性质，并在培育灵芝草调查的替代品的可行性木材日志。糖肽提取灵芝子实体再进行透析隔离。该组件的糖和糖肽的分子量分布用GC和HPLC测定。通过IR检测的糖肽的结构，<'1> H NMR和<'13> C NMR。 GLPW和GLPG的糖肽的分子量为Mw 5.13×10 < '5>和5.85×10 <' 5>，Mη6.21×10 < '5>和6.96×10分别<' 5>。从两个GLPW和GLPG糖肽进行了主要与通过β糖苷键，并且由16种氨基酸以及鼠李糖，的Xyl，FRU Gal和GLC（有在GLPW曼的小的量）。其物理性质和GLPW和GLPG的化学成分非常相似（或具有最小的差异）。 GLPG的糖肽提取率为2.8倍GLPW。

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