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首页> 外文会议>AIChE Annual Meeting >Surface Attachment of α-Chymotrypsin to Polymeric Nanoparticles and Its Kinetic Behaviors Associated with Particle Size
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Surface Attachment of α-Chymotrypsin to Polymeric Nanoparticles and Its Kinetic Behaviors Associated with Particle Size

机译：α-chymotrypsin与聚合物纳米粒子的表面附着及其与粒径相关的动力学行为

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Nanoparticles provide maximum surface area (per unit mass) for surface attachment of enzymes. In addition to high enzyme loading and minimum diffusional limitation, nanoparticle biocatalysts may behave in transitional region between the heterogeneous (with immobilized enzymes) and homogeneous (with soluble free enzymes) catalysis, if considering the unique solution behaviors of the nanoparticles. According to Einstein-Stokes equation and the collision theory, the mobility (size) of the catalyst may impact the reaction kinetics. In the present work, a model enzyme, α-chymotrypsin, was covalently attached to polystyrene nanoparticles with sizes (diameters) ranging from 110 nm to 1000 nm. An active enzyme loading up to 6.6 wt% was achieved as determined by active site titration. It appeared that the size of the particle affected the enzyme activity in a way that can be expected from collision theory.
机译：纳米颗粒为酶的表面附着提供最大表面积（每单位质量）。除了高酶负载和最小漫射限制之外，如果考虑纳米颗粒的独特溶液行为，纳米颗粒生物催化剂可能在非均相（具有固定化酶）和均匀（具有可溶性游离酶）之间的过渡区域中。根据爱因斯坦 - 斯托克斯方程和碰撞理论，催化剂的迁移率（尺寸）可能影响反应动力学。在本作本作中，模型酶，α-脉键素，共价连接到聚苯乙烯纳米颗粒，其尺寸（直径）为110nm至1000nm。通过活性位点滴定测定，实现高达6.6wt％的活性酶。似乎颗粒的尺寸以碰撞理论的方式影响了酶活性。

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