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首页> 外文会议>AIChE Annual Meeting >COMPARISION OF HYPER-SPECTRAL WIDE-FIELD AND CONFOCAL FLUORESCENCE MICROSCOPIC TECHNIQUES
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COMPARISION OF HYPER-SPECTRAL WIDE-FIELD AND CONFOCAL FLUORESCENCE MICROSCOPIC TECHNIQUES

机译：高光谱宽场和共聚焦荧光显微技术的比较

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Acute lung injury (ALI) is characterized by alterations in epithelial and endothelial cell function, pulmonary edema, and atelectasis. Pulmonary microvascular endothelial cells (PMVECs) are believed to play an important role in reconstructing the damaged endothelium during lung injury. Hence, there is a need to track PMVECs to understand their role in, reforming damaged endothelium. Fluorescent protein expression is a potentially powerful tool for labeling and tracking these cells. However, attempts to detect fluorescently-labeled cells in lung tissue using standard single-wavelength fluorescence microscopy approaches have been unsuccessful, due to the high autofluorescence of the pulmonary vasculature and surrounding lung tissues.
机译：急性肺损伤（ALI）的特征在于上皮和内皮细胞功能，肺水肿和ATELECTAS的改变。据信肺微血管内皮细胞（PMVEC）在重建肺损伤期间重建受损内皮的作用起着重要作用。因此，需要跟踪PMVEC以了解其在重新损坏内皮中的作用。荧光蛋白表达是一种潜在的标记和跟踪这些细胞的强大工具。然而，由于肺脉管系统和周围肺组织的高自发性，试图使用标准单波长荧光显微镜方法检测肺组织中肺组织中的荧光标记的细胞。

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