• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>NSTI Nanotechnology Conference and Trade Show >Cell-based Assays for Cytotoxic and Pro-inflammatory Effects of Gold Nanoparticles
【24h】

Cell-based Assays for Cytotoxic and Pro-inflammatory Effects of Gold Nanoparticles

机译：基于细胞的细胞毒性和金纳米粒子的促炎作用的测定

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nanoparticles are widely used in industrial products and have great potential in medical applications, however, their toxicity and effects on health are unknown. This study has explored the in vitro cytotoxic and proinflammatory effects of 15 nm gold nanoparticles. Little to no cytotoxicity was observed in RAW264.7 murine macrophages, Jurkat T lymphocytes, RAJI B lymphocytes, and WHTBF-6 lung fibroblasts after 24 hour treatment with up to 2.5 μg/cm{sup}2 nanoparticles. The ability for RAW264.7 cells to internalize fluorescent latex beads was not significantly inhibited by 24 hr treatment with nanoparticles. An increase in the production of the cytokine TNF-α was observed in RAW264.7 cells after treatment with 2.5 μg/cm{sup}2 nanoparticles. TNF-α levels in freshly isolated human peripheral blood mononuclear cells were elevated after treatment with 1.25, 2.5 and 5.0 μg/cm{sup}2 nanoparticles. An increase in phosphorylation of the MAP kinase proteins SAPK and JNK, was observed in RAW264.7 cells after treatment with 0.25 to 2.5 μg/cm{sup}2 nanoparticles.
机译：纳米粒子广泛用于工业产品，在医疗应用中具有很大的潜力，然而，它们的毒性和对健康的影响是未知的。本研究探索了15nM金纳米颗粒的体外细胞毒性和促炎作用。在RAW264.7小鼠巨噬细胞中观察到很少或没有细胞毒性，的Jurkat T淋巴细胞，RAJI B淋巴细胞，和WHTBF-6肺成纤维细胞后，用高达2.5微克/平方厘米{SUP} 2个纳米颗粒处理24小时。用纳米颗粒的24小时处理没有显着抑制对Raw264.7细胞的能力。在RAW264.7细胞中观察到治疗后在生产细胞因子TNF-α的增加，用2.5微克/厘米{SUP} 2个纳米颗粒。在新鲜分离的人外周血单核细胞TNF-α水平处理后升高与1.25,2.5和5.0微克/厘米{SUP} 2个纳米颗粒。在MAP的磷酸化的增加激酶SAPK和JNK，在RAW264.7细胞中观察到的蛋白处理后，用0.25至2.5微克/平方厘米{SUP} 2个纳米颗粒。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号