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首页> 外文会议>Saratov Meeting >Soluble guanylyl cyclase is involved in PDT-induced injury of crayfish glial cells
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Soluble guanylyl cyclase is involved in PDT-induced injury of crayfish glial cells

机译：可溶性观音环酶参与PDT诱导的小龙虾胶质细胞损伤

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Photodynamic therapy (PDT) is a potential tool for selective destruction of malignant brain tumors. However, not only malignant but also healthy neurons and glial cells may be damaged during PDT. Nitric oxide is an important modulator of cell viability and intercellular neuroglial communications. NO have been already shown to participate in PDT-induced injury of neurons and glial cells. As soluble guanylyl cyclase is the only known receptor for NO, we have studied the possible role of soluble guanylyl cyclase in the regulation of survival and death of neurons and surrounding glial cells under photo-oxidative stress induced by photodynamic treatment (PDT). The crayfish stretch receptor consisting of a single identified sensory neuron enveloped by glial cells is a simple but informative model object. It was photosensitized with alumophthalocyanine photosens (10 nM) and irradiated with a laser diode (670 nm, 0.4 W/cm2). Using inhibitory analysis we have shown that during PDT soluble guanylyl cyclase, probably, has proapoptotic and antinecrotic effect on the glial cells of the isolated crayfish stretch receptor. Proapoptotic effect of soluble guanylyl cyclase could be mediated by protein kinase G (PKG). Thus, the involvement of NO/sGC/cGMP/PKG signaling pathway in PDT-induced apoptosis of glial cells was indirectly demonstrated.
机译：光动力治疗（PDT）是一种选择性破坏恶性脑肿瘤的潜在工具。然而，在PDT期间，不仅是恶性而且健康的神经元和胶质细胞可能会受损。一氧化氮是细胞活力和细胞间神经元素通信的重要调节剂。没有已经显示出参与PDT诱导的神经元和胶质细胞损伤。随着可溶性的冠状阴性环酶是唯一已知的受体，我们已经研究了可溶性观光环酶在光动力学处理（PDT）诱导的光氧化应激下的神经元和周围胶质细胞的调节中的可能作用。由胶质细胞包围的单个鉴定的感官神经元组成的小龙虾拉伸受体是一个简单但信息丰富的模型对象。用铝酞菁（10nm）光敏化，并用激光二极管（670nm，0.4w / cm 2）照射。使用抑制分析我们已经表明，在PDT可溶性观论过程中，可能对孤立的小龙虾拉伸受体的神经胶质细胞具有促凋亡和抑制作用。可溶性观光环酶的促液凋亡效应可以通过蛋白激酶G（PKG）介导。因此，间接证明了NO / SGC / CGMP / PKG信号传导途径在PDT诱导的胶质细胞凋亡中的累积。

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