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Spectral transmission by the specimens of blood-glucose mixture

机译：血糖混合物标本的光谱传输

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Influence of glucose addition to specimens of diluted blood on the transmission spectrums have been investigated by experimentally and by the simulation. The coefficients of extinction, scattering and absorption of erythrocytes were calculated with using Mie theory for spherical particles of equal volume. Transmission spectrums were measured with the spectrophotometer Cary500 in the wavelength range 460-860 nm. Specimens of liquid for imbedding of erythrocytes were preparing by mixing blood plasma and 50-% glucose solution with the different concentrations. The volume concentrations (hematocrit) of red blood cells (RBC) were maintained to have the same values in all specimens by adding equal volume of whole blood to immersion liquid of equal volumes. It has been shown that transmission is decreasing for all wavelengths with the addition of glucose solution on condition that the volume concentrations less than 0.35-0.4. The subsequent increase of the glucose concentration results in increasing of spectral transmission.
机译：通过实验和模拟研究了葡萄糖补充对稀释血液标记的影响。使用MIE理论计算红细胞的消光，散射和吸收和吸收的红细胞，用于相等体积的球形颗粒。用波长范围460-860nm的分光光度计Cary500测量透射光谱。通过将血浆和50％葡萄糖溶液与不同浓度混合，制备用于嵌入红细胞的液体标本。通过将相等体积的全血加入到相等体积的浸入液体，保持红细胞（RBC）的体积浓度（RBC）在所有标本中具有相同的值。已经表明，在体积浓度小于0.35-0.4的条件下，透射率随着葡萄糖溶液的增加而降低了所有波长。随后的葡萄糖浓度的增加导致频谱传输的增加。

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