Investigation of quantum dot FRET in the far-red spectral region

机译：远红光谱区量子点褶的研究

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Colloidal quantum dots (QDs) are now commercially available in a bio-functionalized form and F?rster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed by several groups of researchers. We are particularly interested in the far-red region, as from a biological perspective, there are benefits in pushing to ~700 nm to minimize optical absorption (ABS) within tissue and avoiding cell autofluorescence. We report on FRET between streptavidin (STV) conjugated CdTe quantum dots, Qdot705-STV, with biotinylated Dy731-Bio fluorescent molecules in a donor-acceptor assay. We also highlight an unusual change in Dy731-Bio absorptivity during the streptavidin-biotin binding process that can be attributed to the structural reorientation. In moving to wavelengths beyond 700 nm, different alloy compositions are required for the quantum dot core and these introduce associated changes in the physical shape. These changes directly affect the fluorescence decay dynamics producing a marked biexponential decay with an extremely long lifetime component, a lifetime in excess of 100 ns. We compare and contrast the influence of the two QD relaxation processes upon the FRET dynamics in the presence of Dy731-Bio.

机译：现在，几组研究人员，胶体量子点（QDS）现在以生物官能化的形式（Bio官能化的形式和B生物缀合的点和荧光团之间的F 2螺母共振能量转移（FRET）。我们对远红地区特别感兴趣，因为从生物学的角度来看，推动〜700nm有益处，以最小化组织内的光学吸收（ABS）并避免细胞自发荧光。在供体 - 受体测定中，我们在链霉抗生物素蛋白（STV）共轭CDTE量子点，qDOT705-STV之间的QDOT705-STV之间的FRET报告。在链霉抗生物素蛋白 - 生物素结合过程中，我们还突出了DY731-BIO吸收率的不寻常的变化，其可归因于结构重新定位。在移动到超过700nm的波长时，量子点芯需要不同的合金组合物，并且这些含有不同的变化。这些变化直接影响产生具有极长的寿命部件的标记的Biexponential衰减的荧光衰减动态，寿命超过100ns。我们对照在DY731-BIO存在下，对两种QD松弛过程的影响进行比较。

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《Conference on Colloidal Quantum Dots for Biomedical Applications》|2007年||共10页
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E. Z. Chong; D. R. Matthews; H. D. Summers; K. L. Njoh; R. J. Errington; P. J. Smith;
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中图分类光、激光生物医学;
关键词
Quantum dot; FRET; far-red; fluorophore; streptavidin; biotin; lifetime; CdTe; CdSe;

机译：量子点;FRET;远红;荧光团;链霉抗生物素蛋白;生物素;寿命;CDTE;CDSE;

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专利

1. Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots [J] . E. Z.Chong, D. RMatthews, H. D.Summers, BioMed research international . 2007,第5期

机译：使用CdTe量子点在远红外中基于FRET的检测方法的开发
2. A ratiometric and far-red fluorescence "off-on" sensor for sequential determination of copper(II) and L-histidine based on FRET system between N-acetyl-L-cysteine-capped AuNCs and N,S,P co-doped carbon dots [J] . Mikrochimica Acta: An International Journal for Physical and Chemical Methods of Analysis . 2020,第5期

机译：用于顺序测定铜（II）和L-组氨酸的铜（II）和L-组氨酸的铜（II）和L-组氨酸的比例和远红色荧光“OFF-ON”传感器在N-乙酰基-1-半胱氨酸封端的AUND和N，S，P共掺杂碳之间进行顺序测定铜（II）和L-组氨酸小点
3. A far-red FRET fluorescent probe for ratiometric detection of L-cysteine based on carbon dots and N-acetyl-L-cysteine-capped gold nanoparticles [J] . Dong Wenjuan, Wang Ruiping, Gong Xiaojuan, Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2019,第期

机译：基于碳点和N-乙酰-1-半胱氨酸封闭金纳米粒子的型L-半胱氨酸的比率检测远红发荧光探针
4. Investigation of quantum dot FRET in the far-red spectral region [C] . E. Z. Chong, D. R. Matthews, H. D. Summers, Colloidal Quantum Dots for Biomedical Applications II; Progress in Biomedical Optics and Imaging; vol.8 no.25; Proceedings of SPIE-The International Society for Optical Engineering; vol.6448 . 2007

机译：远红外光谱区量子点FRET的研究
5. Spatially- and spectrally-resolved investigations of indium arsenide quantum dot structures. [D] . Popescu, Paul Dan. 2003

机译：砷化铟量子点结构的空间分辨和光谱分辨研究。
6. Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots [O] . E. Z. Chong, D. R. Matthews, H. D. Summers, 2007

机译：使用CdTe量子点在远红外中基于FRET的检测方法的开发
7. Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots [O] . Chong, E. Z., Matthews, D. R., Summers, H. D., 2007

机译：使用CdTe量子点在远红外中基于FRET的检测方法的开发

Investigation of quantum dot FRET in the far-red spectral region

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