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Step-feeding Biological Contact Oxidation Process and Its Microbial Community Structure for Treating Polluted River Water in the Dianchi Lake Watershed

机译:养育生物接触氧化过程及其微生物群落结构,用于治疗滇池流域污染河水

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The Dianchi Lake receives most of the pollution load from 29 rivers in the watershed.To control lake eutrophication,it is a cost-effective measure to split and purify some river flow in a bypass treatment system.A demonstration work of step-feeding biological contact oxidation process (SBCOP) with the treatment scale of 1000 m3/d for river water treatment was built and operated on the bank of Daqing River,the most seriously polluted river in the Dianchi Lake watershed.Experiments were carried out to determine the treatment efficiency of the SBCOP demonstration work,and the microbial community structures of biofilm and sludge in the SBCOP demonstration work were also analyzed by using denaturing gradient get electrophoresis (DGGE) technique to verify the treatment efficiency.The hydraulic retention time (HRT) was 4.75 h in the whole operation period.According to the influent qualities and climatic conditions,the step-feeding ratio (4∶3∶2 and 1∶1∶1) and air-water rate (3∶1 and 2∶1) were adjusted.The results showed that SBCOP demonstration work kept high removal efficiencies for COD and NH4+-N,and the average removal rates were 37.7% and 32.9% each; while the average removal rates of TN and TP were as low as 10.5% and 13.7% each.The microbial community structure experienced great changes during the whole operation,and the temporal change had more significant effect on microbial communities in treatment systems than spatial change.Biofilm,sludge,Spirogyra sp.,and duckweed were composed of a complex microcosm which could resist the environmental changes.When the influent was lake water,the DNA band numbers and Shannon-winner Indexes of microbial community got lower,possibly due to the shortage of substrate.The results of DNA sequencing indicated that the microorganisms with known sequences were mainly uncultured ones.The dominant microorganisms in the SBCOP demonstration work included Proteobacteria (α,ε,and γ-Proteobacteria),Bacteroides and unknown groups.Based on the sources in the NCBI database,all the dominant microorganisms are helpful to the purification of contaminants,and some of them are denitrifying bacterial strains.
机译:Dianchi Lake从流域中的29河中获得大部分污染负荷。控制湖富营养化,这是一种经济有效的措施,可在旁路处理系统中分裂和净化一些河流。饲养生物接触的示范工作氧化过程(SBCOP)为1000 M3 / d的河水处理的治疗规模被建造并在大庆河岸上建造并运营,这是滇池流域最严重污染的河流。进行了实验,以确定治疗效率通过使用变性梯度进行电泳(DGGE)技术,还通过使用变性梯度进行了电泳(DGGE)技术,分析了SBCOP示范工作和SBCOP示范工作中的生物膜和污泥的微生物群落结构,以验证治疗效率。液压保留时间(HRT)为4.75小时整个运营期。根据影响的品质和气候条件,饲养比率(4:3:2和1:1:1)和空水速率(3:1和2:1)调整结果表明,SBCOP演示工作保持高鳕鱼和NH4 + -N的效率高,平均除去率为37.7%和32.9%;虽然TN和TP的平均除去率低至10.5%和13.7%。微生物群落结构在整个操作期间经历了巨大的变化,并且时间变化对治疗系统中的微生物社区的影响比空间变化更大。生物膜,污泥,螺霉素Sp。和浮萍是由一种复杂的微观组成,可以抵抗环境变化。在水域湖水中,微生物群落的DNA频段数量和Shannon-Winner指数较低,可能是由于短缺DNA测序结果表明,具有已知序列的微生物主要是未培养的序列。SBCOP示范工作中的主要微生物包括植物(α,ε和γ-植物),菌株和未知组。基于来源在NCBI数据库中,所有主要的微生物都有助于污染物的纯化,其中一些是反硝化细菌菌株。

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