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首页> 外文会议>International forum on post-genome technologies >CONSTRUCTION, EXPRESSION AND CHARACTERIZATION OF CHIMERA OLFACTORY RECEPTOR
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CONSTRUCTION, EXPRESSION AND CHARACTERIZATION OF CHIMERA OLFACTORY RECEPTOR

机译：Chimera嗅觉受体的构建，表达和表征

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Animals can recognize various chemical compounds by olfactory system. Olfactory cells are equipped with olfactory receptors (ORs), which locate on the cell membranes and bind to chemical compounds specifically. We have been trying to construct chemical sensors by expressing ORs in Saccharomyces cerevisiae. We constructed a chimera olfactory receptor of rat 17 olfactory receptor and mouse mOR226, which recognizes 2, 4-dinitrotoluene (DNT), analogue of trinitrotoluene. Alpha factor receptor, the authentic GPCR, was replaced by the chimera receptor, and GFP or firefly luciferase was introduced as a reporter gene. Introduction and expression of the chimera OR gene was confirmed by PCR and RT-PCR. Then, production of chimera OR was confirmed by western blotting. Finally, we examined the effect of addition of 2, 4-DNT. The transformed yeast cells exhibited dose dependent bioluminescence.
机译：动物可以通过嗅觉系统识别各种化合物。嗅觉细胞配备有嗅觉受体（ORS），其定位在细胞膜上并具体结合化学化合物。我们一直试图通过在酿酒酵母中表达或在酿酒酵母中构建化学传感器。我们构建了大鼠17嗅觉受体和小鼠Mor226的嵌合膜嗅受体，其识别出2,4-二硝基甲苯（DNT），三立化甲苯的类似物。 α因子受体，真正的GPCR，被嵌合受体所取代，并将GFP或萤火虫荧光素酶作为报告基因引入。通过PCR和RT-PCR确认嵌合体或基因的引入和表达。然后，通过Western印迹确认嵌合体或确认。最后，我们检查了添加2,4-DNT的效果。转化的酵母细胞表现出剂量依赖性生物发光。

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