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首页> 外文会议>International forum on post-genome technologies >MGMT PROMOTER METHYLATION IN ESOPHAGEAL CANCER CELL LINES AND ITS EFFECT ON TRANSCRIPTIONAL ACTIVITY
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MGMT PROMOTER METHYLATION IN ESOPHAGEAL CANCER CELL LINES AND ITS EFFECT ON TRANSCRIPTIONAL ACTIVITY

机译：食管癌细胞系中MgMT启动子甲基化及其对转录活性的影响

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Objective: To investigate the status of methylation in MGMT promoter region in seven esophageal cancer cell lines and their expression level of MGMT mRNA and to explore the possible mechanism of MGMT transcriptional loss. Methods: Methylation specific PCR (MSP) and quantitative real-time reverse transcription PCR(RT-PCR) were introduced to detect the status of methylation and the mRNA expression level of MGMT ; simultaneously, dual-luciferase reporter assay system was established to confirm the transcriptional inactivation of MGMT due to its promoter hypermethylation of CpG islands, Results: Two esophageal cancer cell lines: KYSE - 150 and KYSE -510 had a high status of methylated promoter and a low expression of MGMT mRNA compared to the other five cell lines and the hypermethylation of CpG islands gave rise to this transcriptional silencing. Conclusion: The hypermethylation of MGMT CpG islands blocked its transcriptional activity and the analysis of the methylation status could be a new technology to prognose the happenings of esophageal cancer.
机译：目的：探讨甲基化MGMT启动子区在七个食道癌细胞系中的地位和MGMT的mRNA的表达水平，并探讨MGMT转录损失的可能机制。方法：甲基化特异性PCR（MSP）和被引入检测甲基化的状态，并MGMT的mRNA表达水平的定量实时逆转录PCR（RT-PCR）;同时，双荧光素酶报告测定系统的建立是为了确认MGMT的转录失活因的CpG岛，结果它的启动子甲基化：两个食道癌细胞系：KYSE - 150和-510 KYSE过甲基化启动子和高状态与其他五种细胞系相比，MgMt mRNA的低表达和CpG岛的高甲基化产生该转录沉默。结论：MGMT CPG岛的高甲基化阻断了其转录活性，分析甲基化状态可能是一种新技术，以预先对食管癌发生的事件进行预测。

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