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首页> 外文会议>International symposium on medical and pharmaceutical biotechnology >Design and prokaryotic expression of a multi-epitope assembly peptides (MAP) derived from herpes simplex virus type Ⅱ glycoprotein
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Design and prokaryotic expression of a multi-epitope assembly peptides (MAP) derived from herpes simplex virus type Ⅱ glycoprotein

机译：衍生自单纯疱疹病毒Ⅱ型糖蛋白的多表位组装肽（MAP）的设计和原核表达

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To design a Multi-Epitope Assembly Peptides (MAP) derived from herpes simplex virus type II glycoprotein( HSV2), cloning and fusion expression of the peptides in Escherichia coli by genetic engineering technology. B cell Antigenic epitope analysis, on glycoproteins from HSV2, was based on related software. CD4+T cell Antigenic epitope analysis, on glycoproteins from HSV2, based on other related software. The whole eleven epitopes were connected by linker. Spatial structure of the random Assembly Peptides was predicted by the software. The selected sequence was chemically synthesized and cloned into and inserted into the E. coli expression vector pET27b( + ). The epitope independence of assembly was designed successfully, SDS-PAGE showed that a band corresponding to a protein of 33 kDa was obtained. The way of obtaining spatial structure of MAP by the sofeware was feasible, The sequence of recombinant protein may be applied for further study for the function of candidates vaccine of HSV2.
机译：为了设计从单纯疱疹病毒II型糖蛋白（HSV2），克隆和通过基因工程技术在大肠杆菌中肽的融合表达衍生的多表位肽大会（MAP）。 B细胞抗原表位分析，从HSV2的糖蛋白，是根据相关软件。 CD4 + T细胞抗原表位分析，从HSV2的糖蛋白，基于其他相关软件。整个11个表位的接头连接。随机大会肽的空间结构是由软件预测。所选择的序列化学合成并克隆到并插入到大肠杆菌表达载体pET27b（+）。组装该表位的独立性被成功地设计，SDS-PAGE表明获得对应于33 kDa的蛋白条带。由sofeware获得MAP的空间结构的方式是可行的，重组蛋白的序列可以被应用于用于HSV2的候选疫苗的功能进一步研究。

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