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Mitochondria-dependent apoptosis in HeLa cell induced by methylene blue-mediated photodynamic therapy

机译:亚甲基蓝介导的光动力疗法诱导的HeLa细胞中的线粒体依赖性细胞凋亡

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Methylene blue (MB), a widely studied reagent, is investigated in this work for its usage in photodynamic therapy (PDT). PDT has been proved to be highly effective in the treatment of different types of cancers. To elucidate the effects of MB in PDT, we analyzed PDT-induced apoptosis in HeLa cells by introducing different doses of MB into the culture media. Our data showed that MB-mediated PDT triggered intense apoptotic cell death beginning with photochemical generation of reactive oxygen species. The release of cytochrome c and activation of caspase-3 indicated that MB-PDT-mediated apoptosis in HeLa cells was executed by the mitochondria-dependent apoptotic pathway.
机译:在这项工作中,研究了亚甲基蓝(MB),是一种广泛研究的试剂,用于其在光动力治疗(PDT)中的用途。 PDT已被证明在治疗不同类型的癌症时非常有效。为了阐明MB在PDT中的影响,通过将不同剂量的MB引入培养基,在Hela细胞中分析了PDT诱导的凋亡。我们的数据显示,MB介导的PDT引发激烈的凋亡细胞死亡,从反应性氧物种的光化学产生开始。细胞色素C的释放和Caspase-3的激活表明,由线粒体依赖性凋亡途径执行MB-PDT介导的HeLa细胞的细胞凋亡。

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