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Growth Rate Model and Temperature-changing Fermentation Process of Pseudomonas syringae pv. mori M4-13

机译:PSEUDOMONAS Syringae PV的生长速率模型和温度变化发酵过程。森M4-13

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Pseudomonas syringae pv.mori M4-13 is a new coronatine-production strain isolated from mulberry trees. As a high efficient plant growth substance, coronatine is difficult to obtain from the traditional bacteria under the high temperature. The fermentation temperature cannot be greater than 301K. However, the coronatine production is strictly growth associated. Therefore, biomass growth and accumulation of coronatine should be studied coordinately. In this paper, the growth rate of the strain was studied by the square root model, and the temperature-changing fermentation pattern of coronatine was optimized. In the fitting function of k=[b(T-T_(min))]~2{1-exp[c(T-T_(max))]} the value of b was 0.03276, c was 0.1759, R~2= 0.99. Based on the results, the optimal growth temperature of Pseudomonas syringae pv. mori M4-13 is 305K. The accumulation of coronatine reaches the peak, when the strain was incubated at the 305K for 3 days, following with the fermentation at 291K for another 3days. This fermentation pattern lay a solid foundation for the large-scale applications in the industrial production.
机译:Pseudomonas syringae pv.mori m4-13是一种从桑树孤立的新冠状素 - 生产菌株。作为一种高效的植物生长物质,核素难以在高温下从传统细菌中获得。发酵温度不能大于301K。然而,核素生产是严格的增长相关。因此,应协调地研究生物量生长和核素的积累。在本文中,通过平方根模型研究了菌株的生长速率,优化了冠状胺的温度变化的发酵模式。在k = [b(t-t_(min))]〜2 {1-exp [c(t-t_(max))]} B的值为0.03276,c为0.1759,R〜2 = 0.99。基于结果,Pseudomonas Syringae PV的最佳生长温度。 Mori M4-13是305k。当在305K在305K温育3天时,冠状酮的积累达到峰,在291K持续291K的发酵后3天。这种发酵模式为工业生产中的大规模应用奠定了坚实的基础。

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