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Toxicity and Uptake Kinetics of Pentachlorophenol on the Eisenia fetida Exposed to Test Soils

机译:五氯苯酚对艾西尼亚艾丽达暴露于测试土壤的毒性和摄取动力学

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Pentachlorophenol (PCP) is widely distributed and highly persistent in soil, and represents a threat to the health of ecosystems. The present study aimed to assess the toxicity, bioavailability and uptake kinetics by earthworms on PCP in soils as a function of different aging periods with the attempt to select a good uptake kinetic. The results show that body residues in the controls were significantly different from all the body residues. The bioconcentration factor increased with time at both PCP loadings, the neutral red retention time for earthworms was reduced with increasing exposure concentration of PCP since 28 days of aging, and the NRRT assay was highly sensitive to PCP in E. fetid a lysosomal and appearing decisively more sensitive than the other applied assays, the lysosomal membrane stability may therefore be a useful biomarker tool to estimate toxic effects of PCP on earthworms. The uptake kinetics of PCP in test soil by earthworms could be well described by the uptake kinetics model, with increased aging time, the bioavailability of PCP increased and in theory the rate constants calculated in uptake and elimination studies are indeed constants and can be used to predict accumulation in earthworm. The earthworm uptake PCP demonstrated the enhanced availability of PCP with LMWOA addition, this provides an information to enhance availability of PCP by organic acids.
机译:五氯苯酚(PCP)广泛分布在土壤非常执着,而且代表了生态系统的健康构成威胁。本研究的目的是评估毒性,生物利用度和摄取由PCP蚯蚓动力学在土壤中作为与尝试选择好摄取动力学不同老化周期的函数。结果表明,体内残留的控制是所有身体残留显著不同。生物浓度因子在两个PCP载荷随时间增加,中性红保留时间与蚯蚓PCP暴露浓度的增加,因为老化28天被减小,并且NRRT测定是高度灵敏的,以在PCP E.恶臭溶酶体和果断地出现超过了其他应用的测定敏感,溶酶体膜稳定性可能因此是估计PCP的对蚯蚓的毒性作用的有用的生物标志物的工具。在测试土壤中的蚯蚓PCP的吸收动力学可以通过摄取动力学模型很好地描述,增加时效时间,PCP的生物利用度增加,在理论上的吸收和消除的研究计算的速率常数的确是常数,可以用来预测蚯蚓积累。摄取PCP蚯蚓证明PCP与LMWOA加入增强的可用性,这提供了一种信息由有机酸以提高PCP的可用性。

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