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首页> 外文会议>International Conference on Human Health and Biomedical Engineering >AcSDKP inhibits the proliferation and collagen expression of cardiac fibroblasts induced by PDGF through blocking the ERK1/2 and JNK pathway activation
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AcSDKP inhibits the proliferation and collagen expression of cardiac fibroblasts induced by PDGF through blocking the ERK1/2 and JNK pathway activation

机译：ACSDKP通过阻断ERK1 / 2和JNK途径激活，抑制PDGF诱导的心肌成纤维细胞的增殖和胶原表达

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In the present study, we investigated the effect of ERK1/2 and JNK signaling pathway in the inhibition of AcSDKP on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts induced by PDGF. By using MTT assay and Western Blot analysis, we found that treating the fibroblasts with PDGF, phospho-ERK1/2 and phosphoJNK expression was significantly increased compared with the control group, while proliferation of cardiac fibroblasts and type I and type Ш collagen expression also increased. However, after PD98059 and SP600125 blocked activation of ERK1/2 and JNK signaling pathway, the effect of PDGF on fibroblasts decreased. Similarly, AcSDKP also significantly inhibited PDGF-induced ERK1/2 and JNK phosphorylation, and at the same time, fibroblasts proliferation and collagen expression induced by PDGF also decreased significantly. These findings suggest that AcSDKP could inhibit the proliferation and collagen expression of cardiac fibroblasts induced by PDGF through blocking the ERK1/2 and JNK pathway activation.
机译：在本研究中，我们研究了ERK1 / 2和JNK信号通路在PDGF诱导的培养大鼠心脏成纤维细胞中抑制ACSDKP的抑制作用。通过使用MTT测定和Western印迹分析，我们发现与对照组相比，用PDGF处理成纤维细胞，磷酸-ERK1 / 2和磷酸杂交表达显着增加，而心肌成纤维细胞和I型和Ⅳ型胶原表达的增殖也增加了。然而，在PD98059和SP600125堵塞ERK1 / 2和JNK信号通路的激活后，PDGF对成纤维细胞的影响降低。类似地，ACSDKP也显着抑制了PDGF诱导的ERK1 / 2和JNK磷酸化，同时，PDGF诱导的成纤维细胞增殖和胶原蛋白表达也显着降低。这些发现表明，ACSDKP可以通过阻断ERK1 / 2和JNK途径激活来抑制PDGF诱导的心肌成纤维细胞的增殖和胶原表达。

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