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Effect of transplantation of bone marrow mesenchymal stem cells on the expression of nerve growth factor and brain-derived neurotrophic factor in rats after sciatic nerve crush injury

机译:骨髓间充质干细胞移植对坐骨神经压碎损伤后大鼠神经生长因子和脑源神经营养因子表达的影响

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To investigate the effect of transplantation of bone marrow mesenchymal stem cells on the expression of nerve growth factor and brain-derived neurotrophic factor in rats after sciatic nerve crush injury, a randomized controlled animal study was performed. Rats in the experimental group underwent transplantation of bone marrow mesenchymal stem cells at sciatic nerve crushed sites. Distal nerve to crushed site suffered from nerve growth factor and brain-derived neurotrophic factor immunohistochemical staining postoperation. Image analysis system was adopted to analyze integrated optical density of positive expression. And several methods were done to confirm that transplantation of stem cells can promote the peripheral nerve regeneration. Nerve growth factor and brain-derived neurotrophic factor protein expression in the experimental group was significantly higher than that in the control group in an early phase after surgery. We draw a conclusion that transplantation of bone marrow mesenchymal stem cells at nerve crushed site can affect the expression of nerve growth factor and brain-derived neurotrophic factor expression in an early phase, and it may be one of its mechanisms that promote the nerve regeneration.
机译:为了探讨骨髓移植的效果坐骨神经挤压伤后的间充质干神经生长因子和脑源性神经营养因子的表达细胞的大鼠,进行随机对照动物实验。在骨髓的实验组接受移植大鼠骨髓间充质在坐骨神经压碎网站干细胞。远端神经从神经生长因子和脑源性神经营养因子免疫组化染色术后遭受粉碎部位。图像分析系统获得通过分析阳性表达的积分光密度。而几种方法都做了证实,干细胞移植可促进周围神经再生。神经生长因子,并在实验组脑源性神经营养因子的蛋白表达为对照组中比显著高于手术后的早期阶段。我们得出一个结论,即骨髓移植间充质干在神经粉碎部位的细胞会影响早期阶段的神经生长因子和脑源性神经营养因子表达的表达,这可能是其机制,促进神经再生一个。

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