Detection of microorganism nucleic acid based on Surface plasmon resonance sensor

机译：基于表面等离子体共振传感器的微生物核酸检测

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In present investigation, we established an on-line microorganism monitoring method based on SPR biosensor. Combined with asymmetric PCR method, we choose 370nt nucleic acid fragment contain the conserved sequence of Staphylococcus aureus for SPR detect. The detect limit was 13.7 ng/ml. The detection result can be effectually improved when the secondary structure of PCR product was broken by degeneration treatment, compared with Oligonucleotide complementary sequences, the result of PCR product is less effect at detect response and reproducibility.

机译：在目前的调查中，我们建立了基于SPR生物传感器的在线微生物监测方法。结合不对称PCR方法，我们选择370NT核酸片段含有用于SPR检测的金黄色葡萄球菌的保守序列。检测限为13.7 ng / ml。当通过变性处理破坏PCR产物的二次结构，与寡核苷酸互补序列相比，检测结果可以有效提高，与寡核苷酸互补序列相比，PCR产物的结果在检测响应和再现性下效果较小。

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《International Conference on Remote Sensing, Environment and Transportation Engineering》|2011年||共3页
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作者
Yin Gu; Chunyan Wang; Yingjun Tan; Yinghui Li;
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中图分类 TP7-53;
关键词
Surface plasmon resonance; Microorganism; DNA;

机译：表面等离子体共鸣;微生物;DNA;

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1. Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique [J] . Pratima R. Solanki, Nirmal Prabhakar, M. K. Pandey, Biomedical Microdevices . 2008,第5期

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2. Nucleic acid sensor for M-tuberculosis detection based on surface plasmon resonance [J] . Prabhakar N, Arora K, Arya SK, The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2008,第11期

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3. A Surface Plasmon Resonance Sensor Platform Coupled with Gold Nanoparticle Probes for Unpurified Nucleic Acids Detection [J] . Gu Y., Tan Y.-J., Wang C.-Y., Analytical Letters . 2012,第13a15期

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4. Detection of microorganism nucleic acid based on surface plasmon resonance sensor [C] . Yin Gu, Chunyan Wang, Yingjun Tan, 2011 International Conference on Remote Sensing, Environment and Transportation Engineering . 2011

机译：基于表面等离子体共振传感器的微生物核酸检测
5. Ultrasensitive nucleic acid detection using surface plasmon resonance imaging measurements via a novel surface amplification process and microfluidic networks. [D] . Goodrich, Terry T. 2004

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6. Quantitative Analysis of Small Molecule–Nucleic Acid Interactions With a Biosensor Surface and Surface Plasmon Resonance Detection [O] . Yang Liu, W. David Wilson -1

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7. Nucleic acid sensor for M. tuberculosis detection based on surface plasmon resonance [O] . Prabhakar Nirmal, Arora Kavita, Arya Sunil K., 2008

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8. Development of Nucleic Acid Aptamer-Based Sensors for the Direct Detection and Identification of Biological Agents [R] . Stratis-Cullum, D. N. , Gillespie, J. B. 2005

机译：基于核酸适体的传感器在生物制剂直接检测和鉴定中的应用

Detection of microorganism nucleic acid based on Surface plasmon resonance sensor

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