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Isolation, Identification and Characterization of heterotrophic nitrifying bacteria from surface water

机译:地表水异养硝化细菌的分离,鉴定及表征

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For reducing ammonium concentration and guaranteeing safe drinking water, three heterotrophic bacteria were isolated from the Songhua River, which were SFA6, SFA7 and SFA11. When the initial ammonium concentration was about 130 mg/L, the ammonium removal rate of the strains SFA7, SFA6 and SFA 11 were 1.54 mg NH_4~+/L/h, 1.20 mg NH_4~+/L/h and 1.27 mg NH_4~+/L/h respectively at 8 °C. The 16S rDNA sequence results revealed that the strain SFA6 was Bacillus subtilis, SFA7 was Pseudomonas putida, and SFA11 showed similarity to Pseudomonas nitroreducens. The biochemical characteristics of SFA6 were quite different from that of SFA7 and SFA11. After 48 h degradation, the NH_4~+/-N (about 5 mg/L) was consumed to 0.23±0.15 mg/L, 0.37±0.20 mg/Land 0.58±0.17 mg/L by the strains SFA6, SFA7 and SFA11, with little NO_3~--N and NO_2~--N accumulation. Above all, the strains SFA6, SFA7 and SFA11 could be used in drinking water treatment at 8 °C. The strain SFA6 showed the highest ammonium removal efficiency.
机译:为了减少铵浓度并保证安全饮用水,从松花江中分离出三种异养细菌,其是SFA6,SFA7和SFA11。当初始铵浓度约为130mg / L时,菌株SFA7,SFA6和SFA 11的铵去除速率为1.54mg NH_4〜+ / L / H,1.20mg NH_4〜+ / L / H和1.27mg NH_4〜分别在8°C下+ / L / h。 16S rDNA序列结果显示,菌株SFA6是枯草芽孢杆菌,SFA7是假单胞菌,SFA11显示与假单胞菌硝雷罗雷森的相似性。 SFA6的生化特征与SFA7和SFA11的生化特征与SFA7和SFA11完全不同。降解48小时后,NH_4〜+/- N(约5mg / L)被菌株SFA6,SFA7和SFA11的0.58±0.17mg / L.0.37±0.20mg / L.0.37±0.10mg / L.只有no_3〜 - nn和no_2〜 - 累积。最重要的是,菌株SFA6,SFA7和SFA11可用于在8°C下饮用水处理。菌株SFA6显示出最高的铵去除效率。

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