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首页> 外文会议>International Meeting on Chemical Sensors >Towards the detection of static ATP levels above primary PTPRζ-osteoblastic cells and their knock-out mutants by ATP microbiosensors
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Towards the detection of static ATP levels above primary PTPRζ-osteoblastic cells and their knock-out mutants by ATP microbiosensors

机译：通过ATP微生物传感器检测原发性PTPRζ-成骨细胞及其敲除突变体的静态ATP水平

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摘要

Adenosine-5'-triphosphate (ATP) holds a significant role as omnipresent energy source and as autocrine and paracrine signaling molecule in many cells such as lung cells and bone cells [1]. ATP is considered to the involved in the mechanical stress response of bone cells such as bone-resorbing osteoblast cells or receptor-proteine-tyrosine-phosphatase-zeta (PTAPRζ)-osteoblastic cells [2], which are involved in bone formation, bone regeneration and the control of bone volume. ATP release stimulates the proliferation of these P2 - receptor cell types [3,4]. The "deficient" knock-out mutant behaves different in proliferation and differentitation and thus the ATP release above these cells is expected to be altered. A localized detection of ATP at the cellular level is therefore of significant importance. Using amperometric. ATP mirobiosensors with diameters ranging from 10 - 50 μm enables localized ATP measurements above wild type and knock-out cells.
机译：腺苷-5'-三磷酸（ATP）在许多细胞（如肺细胞和骨细胞）中具有重要作用和作为全分泌能量源和自分泌和帕拉卡碱信号分子[1]。 ATP被认为是骨细胞的机械应力响应所涉及的骨髓细胞或受体 - 蛋白质 - 酪氨酸 - 磷酸酶 - Zeta（PTaPrζ） - 抑制骨髓细胞[2]，其参与骨形成，骨再生 并控制骨体积。 ATP释放刺激这些P2受体细胞类型的增殖[3,4]。 “缺陷”敲除突变体在增殖和鉴别方面的表现不同，因此预计这些细胞的ATP释放将被改变。 因此，在细胞水平处对ATP的局部检测非常重要。 使用安培数。 直径为10-50μm的ATP Mirobios传感器使野生型和敲除细胞高于局部ATP测量。

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