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首页> 外文会议>International Meeting on Chemical Sensors >Real-time monitoring of cell response to drug stimulation by 2D-SPR Sensor: An approach to study neuronal differentiation
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Real-time monitoring of cell response to drug stimulation by 2D-SPR Sensor: An approach to study neuronal differentiation

机译：2D-SPR传感器的药物刺激的细胞响应的实时监测：一种研究神经元分化的方法

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There is a growing demand for the development of new bio-analytical technique that is capable of monitoring neuronal differentiation non-invasively, in real-time and without any fluorescent or radioactive probes. To fulfill these promises, efficient and easy protocols to monitor neuronal differentiation have to be developed. In this study, we demonstrated a rapid and label-free detection method to monitor neuronal differentiation by high-resolution 2D-surface plasmon resonance sensing technology with a CCD camera to analyzing refractive index change of PC12 cells [1]. We have successfully monitored the intracellular translocation of PKC in PC12 cells by 2D-SPR and found that the cells treated with differentiation factor, nerve growth factor (NGF) showed a remarkable enhancement of SPR response to muscarine and carbacol stimulation. Our results suggested that 2DSPR imager might be applicable to monitor neuronal differentiation and to study the expression state of the specific receptors in living state.
机译：对新的生物分析技术的发展需求不断增长，该技术能够实时和没有任何荧光或放射性探针在无侵入性地监测神经元分化。为了满足这些承诺，必须开发出于监测神经元分化的高效和简便的协议。在这项研究中，我们证明了一种快速和无标签的检测方法，通过使用CCD相机通过高分辨率2D表面等离子体共振传感技术监测神经元分化，以分析PC12细胞的折射率变化[1]。我们已经成功地通过2D-SPR监测了PKC的PKC细胞内易位，发现用分化因子治疗的细胞，神经生长因子（NGF）表现出对麝香葡萄酒和碳酸刺激的SPR反应显着提高。我们的研究结果表明，2DSPR成像仪可能适用于监测神经元分化，并研究生活状态的特定受体的表达状态。

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