The protoplast preparation of two strains of Saccharamyces cerevisae

机译：两种酵母菌酿酒酵母的原生质体制备

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This paper researched on the condition of protoplast preparation for two strains isolated by myself, selected the different of time, concentration of zymolase and temperature, in the end ,the proper condition was the 0.2mg/ml of zymolase, 30 °C, the time of 40 minutes, the ratio of regeneration was 68.2%.

机译：本文研究了原生质体制备的两个菌株的条件，选择了不同的时间，致酵素酶和温度的不同时间，浓度为0.2mg / ml酵素酶，30°C，时间40分钟，再生的比例为68.2％。

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《International Conference on Biotechnology, Chemical and Materials Engineering》|2013年||共4页
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Chun hai Zhao; Zhi gang Liu;
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中图分类 TB3-532;
关键词
Saccharamyces cerevisae; protoplast; protoplast preparation;

机译：Saccharomyces Cerevisiae;原生质体;原生质体制备;

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2. Preparation of Korean Traditional Alcoholic Beverage (Yakju) by a Protoplast Fusion Yeast Strain Utilizing Starch and its Quality Characteristics [J] . Min No Ju, Sung Wook Hong, Kwan Tae Kim, Korean Journal of Food Science and Technology . 2009,第5期

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3. Visualization of protoplast fusion and quantitation of recombination in fused protoplasts of auxotrophic strains of Escherichia coli. [J] . Dai M, Ziesman S, Ratcliffe T, Metabolic engineering . 2005,第1期

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4. The protoplast preparation of two strains of Saccharamyces cerevisae [C] . Chun hai, Zhao Zhi, gang Liu International conference on biotechnology, chemical and materials engineering . 2013

机译：两种酿酒酵母菌株的原生质体制备
5. STUDIES ON PROTOPLASTS OF CHLORELLA PREPARATION AND UTILIZATION [D] . 山田, 隆 1983

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6. Preparation of Metabolically Active Protoplasts and Particle Preparations from the Blue-Green Alga Phormidium luridum [O] . John Biggins 1967

机译：蓝绿藻Phomidium luridum的代谢活性原生质体的制备和颗粒制剂
7. Isolation Characterization and Fermented Research of High Producing Saccharamyces Cerevisae [O] . Zhaochunhai 2012

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8. Preparation and Regeneration of Protoplasts of 'Colletotrichum gloeosporioides'f. p. 'Aeschynomene' [R] . TeBeest, D. O., Weidemann, G. J. 1992

机译：'Colletotrichum gloeosporioides'f。原生质体的制备和再生。页。 “萌”

The protoplast preparation of two strains of Saccharamyces cerevisae

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