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Survival of LA-MRSA in Dust of Pig and Poultry Barns

机译:La-MRSA在猪和家禽谷仓中的生存

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Dust of pig and poultry barns can he a carrier of culturable livestock associated methicillin resistant Staphylococcus aureus (LA-MRSA).A re-entrainment into pig herds or poultry flocks from a contaminated environment seems to be possihle.However,the survival of LA-MRSA in the animal environment and the transmission pathways are not fully understood.Therefore we tested the survival o culturable LA-MRSA in 20 dust samples of pig barns and in 9 dust samples of poultry barns.All dust samples were stored in sterile glas tubes with cork plugs at 4°C in the dark.The age of analysed pig dust ranged between 18 and 34 months and the age of analyzed poultry dust varied from 10 to 34 months.The detection limit was 100 cfu LA-MRSA/g dust.LA-MRSA was detected again in two pig dust samples after 21 and 32 months and again in 4 poultry dust samples after 10,14,14 and 17 months.The detection rate (0.10)was lower in the aged pig dust samples than in the aged poultry dust samples (0.44),probably due to the in general older pig dust samples.Spa typing of isolates from the stored poultry dust samples showed the same types (t011 and t034)as found in the original samples.In general the results indicate a loss of culturability in the course of time.Nevertheless.the detected long-time survival of LA-MRSA in animal hous dust suggests an increased risk for a re-entrainment into a new herd or a new flock.The results underline the importance of effective cleaning and disinfection measures to avoid a re-entrainment from indoor dust residues and from the outdoor environment.
机译:猪和家禽谷仓的灰尘可以是培养的牲畜相关甲氧西米氏菌耐金黄色葡萄球菌(La-mrsa)。将重新夹带成猪群或从受污染的环境中的家禽群似乎有可能。然而,La-的生存动物环境中的MRSA和传输途径尚未完全明白。因此我们在猪谷仓20个粉尘样本中测试了生存o培养的La-MRSA,并在禽谷仓的9个粉尘样品中。所有的粉尘样品都储存在无菌Glas管中在黑暗中,软木塞在4°C。分析的猪尘的年龄在18至34个月之间,分析的家禽粉尘的年龄从10到34个月不同。检测限为100 cfu la-mrsa / g dust.la在21至32个月后再次在两只猪除尘样品中检测到-MRSA,再次在10,14,14和17个月后再次在4个家禽粉尘样品中再次检测到4个家禽粉尘样品。检测率(0.10)在老年的猪粉尘样品中较低,而不是老年人家禽粉尘样本(0.44),可能是由于普通较旧的猪粉尘样品。从储存家禽样品中分别键入分离物,显示出在原始样品中的相同类型(T011和T034)。一般结果表明在一段时间内的培养性丧失。无论如何。在动物尘埃粉尘中检测到La-MRSA的长期存活表明,重新夹具进入新群或新群体的风险增加。结果强调了有效清洁和消毒措施的重要性,以避免重新夹具来自室内灰尘残留物和室外环境。

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