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首页> 外文会议>International congress on animal hygiene >IsrE,a Small Non-coding RNA of Invasion Gene Island(SPI-1)Involved in the Virulence of Salmonella enteritidis
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IsrE,a Small Non-coding RNA of Invasion Gene Island(SPI-1)Involved in the Virulence of Salmonella enteritidis

机译：ISRE，侵袭基因岛的小型非编码RNA（SPI-1）参与沙门氏菌肠炎的毒力

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Small non-coding RNAs (sRNA)of a kind of post-transcriptional regulators in bacteria have excited much interest lately and Salmonella has become a model organism for sRNA research.A small non-coding RNA.IsrE.was first identified in pathogenicity island (SPI-1)of Salmonella typhimurium.However,the regulation function of IsrE in virulence genes is still unknown.To investigate the regulation function of IsrE in virulence.we cloned the isrE gene in the reference strain S.enteritidis 50336,and then constructed the inframe deletion mutant 50336ΔisrE by the λ-Red-based recombination system.Novel target genes were screened by qRT-PCR.The result showed that the deletion of isrE significantly decreased the transcription of both outer membrane protein ompD (25-folds)and flagella subunitf/iC (8.6-folds)compared to those in wild type strain,while the motility of mutant 50336ΔisrE was decreased evidently.In addition,the transcription offimA,sefA,csgA,csgD and sthA in 50336ΔisrE were decreased about 3 to 5 folds.It indicated that IsrE could activate the transcription of ompD and fliC significantly and facilitates the expression of fimA,sefA,csgA,csgD and sthA in S.enteritidis.The mutant strain 50336ΔisrE exhibited an decreased ability to adhere to Caco-2 cells as well as the motility ability compared to the wild type strain.The pathogenicity of 50336ΔisrE was detected by infecting 1-day-old chickens by hypodermic injection.The LD50 of the wild type strain 50336 and the mutant 50336ΔisrE for 1-day-old chickens were 2.809×lO8 cfu and 4.084×lO8 cfu respectively.Our study shows that the deletion of isrE may attenuate the virulence of S.enteritidis in chickens by regulating expression of outer membrane protein OmpD,flagella FliC and fimbriae subunits.
机译：细菌中一类转录后调节剂的小非编码RNA（sRNA）兴奋地兴奋，沙门氏菌已成为SRNA研究的模型生物体。在致病性岛中首先鉴定出小的非编码RNA.isre.was（ SPI-1）沙门氏菌伤寒毒蕈。然而，ISRE在毒力基因中的调节功能仍然未知。研究ISRE在毒力中的调节功能。我们克隆了参考菌株S.ENTISIDIS 50336中的ISRE基因，然后构建了λ-红色的重组系统50336Δisre50336Δisre。通过QRT-PCR筛选Novel靶基因。结果表明，ISRE的缺失显着降低了外膜蛋白OMPD（25倍）和鞭毛亚峰的转录/ IC（8.6倍）与野生型菌株的IC（8.6倍），而突变体50336Δisre的动力显着降低。此外，50336Δisre中的转录免疫因素，SEFA，CSGA，CSGD和STHA减少3至5倍。表明ISRE可以显着激活OMPD和FLIC的转录，促进FIMA，SEFA，CSGA，CSGD和STHA在S.ENTERITIDIS中的表达。突变菌株50336Δisre表现出粘附到CACO的能力下降与野生型菌株相比的2个细胞以及可动力能力。通过皮下注射感染1日龄鸡来检测50336Δisre的致病性。野生型菌株50336的LD50和突变体50336Δisre为1天 - 旧鸡分别为2.809×LO8 CFU和4.084×LO8 CFU。我们的研究表明，ISRE的缺失可以通过调节外膜蛋白OMPD，鞭毛和FIMBRIAE亚基的表达来衰减鸡中的S.Enteritidis的毒力。

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