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Gold nanoparticles-based colorimetric sensor for cysteine detection

机译：基于金纳米颗粒的比色传感器用于半胱氨酸检测

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摘要

A simple, sensitive and selective colorimetric method for the detection of cysteine was demonstrated with unmodified gold nanoparticles (AuNPs) as probes. In this approach, the synthesized AuNPs solution was stabilized by the citrate anions as their repulsion protected the AuNPs from aggregation. Cysteine was added to AuNPs solution and was incubated to react for 3 min. The resulting mixture color changes dramatically from red-purple-blue because cysteine induced the nanoparticle aggregation. These processes were studied and characterized by UV-vis spectroscopy, zeta potential and dynamic light scattering. Several parameters including AuNPs size, reaction time and media pH that governed the analytical performance of the method have been studied in detail and optimized. Under the optimized experimental conditions, cysteine could be selectively detected in a concentration range from 0.1 to 0.6 ppm with a limit of detection as 0.01 ppm at a signal-to-noise ratio of 3. The sensitivity was calculated as 1.474 Abs/ppm. Some common interferents such as Na~+, Cu~(2+), Cl~- and urea showed no interference in the determination of cysteine by using AuNPs.
机译：用未经修改的金纳米粒子（AUNP）作为探针对检测半胱氨酸检测的简单，灵敏和选择性的比色方法。在这种方法中，通过柠檬酸盐阴离子稳定合成的AUNPS溶液，因为它们的排斥保护了来自聚集的剖腹产。将半胱氨酸加入到AUNPS溶液中，并孵育以反应3分钟。由此产生的混合物颜色从红紫色蓝色变化，因为半胱氨酸诱导纳米颗粒聚集。通过UV-Vis光谱，Zeta电位和动态光散射进行了这些方法。已经详细研究了几种参数，包括治理该方法的分析性能的AUNPS尺寸，反应时间和介质pH值。在优化的实验条件下，在0.1至0.6ppm的浓度范围内选择性地检测半胱氨酸，以0.01ppm以3.噪声比为0.01ppm。敏感性计算为1.474 ABS / ppm。一些常见的干扰剂如Na〜+，Cu〜（2+），Cl〜 - 和尿素在使用AUNPS时显示出在半胱氨酸的测定中没有干扰。

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