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首页> 外文会议>International Conference on Advances in Energy, Environment and Chemical Engineering >Molecular Cloning and Structure Analysis Of MpYop1, a Membrane Protein in Monascus purpureus
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Molecular Cloning and Structure Analysis Of MpYop1, a Membrane Protein in Monascus purpureus

机译：Mpyop1，Monascus purpureus的膜蛋白的分子克隆及结构分析

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摘要

In all eukaryotes, the peripheral endoplasmic reticulum (ER) branches out of the nuclear envelope as a membrane network of interconnected tubules and membrane sheets with a single lumen. In yeast, Rtn1p and Yop1p are major, redundant components required to maintain the tubular ER under normal circumstances. In this work, we have investigated the homolog of the yeast Yop1 protein in Monascus purpureus. An M. purpureus cDNA library was constructed, and the MpYop1 cDNA was isolated from the cDNA library by random sequencing method. The cDNA was 1008 bp in length, contains a predicted 516 bp ORF that encodes 171 amino acids, the gene was designated MpYop1. The deduced amino acid sequence showed homology to Yop1 from fungi. The TM topology model of MpYop1 was built, the secondary structure is similar to yeast Yop1p. MpYop1 contains four transmembrane α-helices connected by a cytoplasmic loop and two extracellar loop, both the N and C termini are located in the cytoplasm side of ER membrane. According to functions of Yop1 in Yeast, we implicated that the MpYop1 would involved in stabilization of tubule ER.
机译：在所有真核生物中，外周内质网（ER）将核包膜分支为膜网络作为互连小管和膜板的膜网，具有单个内腔。在酵母中，RTN1P和YOP1P是在正常情况下维持管状ER所需的主要的冗余组件。在这项工作中，我们研究了蒙斯马士武器斯酵母yop1蛋白的同源物。构建了一种M. purpureus cDNA文库，通过随机测序法从CDNA文库中分离出MPYOP1 cDNA。 CDNA的长度为1008bp，含有预测的516bp ORF，其编码171氨基酸，该基因被指定为MPYOP1。推导的氨基酸序列向yop1的同源性来自真菌。构建了MPYOP1的TM拓扑模型，二级结构类似于酵母yop1p。 MPYOP1含有四个通过细胞质回路和两个细胞质环连接的跨膜α-螺旋，N和C Termini都位于ER膜的细胞质侧。根据YOP1在酵母中的功能，我们暗示了MPYOP1将参与小管ER的稳定性。

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