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首页> 外文会议>Conference on Optical Coherence tomography and coherence domain optical methods in biomedicine XXI >Scattering angle resolved optical coherence tomography for in vivo murine retinal imaging
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Scattering angle resolved optical coherence tomography for in vivo murine retinal imaging

机译：用于体内小鼠视网膜成像的散射角度分辨光学相干性断层扫描

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Optical coherence tomography (OCT) retinal imaging contributes to understanding central nervous system (CNS) diseases because the eye is an anatomical "window to the brain" with direct optical access to nonmylenated retinal ganglion cells. However, many CNS diseases are associated with neuronal changes beyond the resolution of standard OCT retinal imaging systems. Though studies have shown the utility of scattering angle resolved (SAR) OCT for particle sizing and detecting disease states ex vivo, a compact SAR-OCT system for in vivo rodent retinal imaging has not previously been reported. We report a fiber-based SAR-OCT system (swept source at 1310 nm ± 65 nm, 100 kHz scan rate) for mouse retinal imaging with a partial glass window (center aperture) for angular discrimination of backscattered light. This design incorporates a dual-axis MEMS mirror conjugate to the ocular pupil plane and a high collection efficiency objective. A muring retina is imaged during euthanasia, and the proposed SAR-index is examined versus time. Results show a positive correlation between the SAR-index and the sub-cellular hypoxic response of neurons to isoflurane overdose during euthanasia. The proposed SAR-OCT design and image process technique offer a contrast mechanism able to detect sub-resolution neuronal changes for murine retinal imaging.
机译：光学相干断层扫描（OCT）视网膜成像有助于了解中枢神经系统（CNS）疾病，因为眼睛是具有直接光学接入非亚lynated视网膜神经节细胞的解剖学“窗口”的解剖学“窗口”。然而，许多CNS疾病与超出标准OCT视网膜成像系统的分辨率的神经元变化相关。虽然研究表明，用于粒度尺寸和检测疾病状态的散射角分辨（SAR）OCT的效用，但先前尚未报道用于体内啮齿动物视网膜显像的紧凑型SAR-OCT系统。我们报告了基于光纤的SAR-OCT系统（以1310nm±65nm，100kHz扫描速率为100 kHz扫描速率），用于鼠标视网膜成像，其具有部分玻璃窗（中心孔径），用于反向散射光的角度辨别。该设计包括双轴MEMS镜像镜像与眼光瞳平面和高收集效率目标。修复视网膜在安乐死期间成像，并检查了所提出的SAR指数与时间。结果表明，在安乐死期间，SAR指数与神经元的亚细胞缺氧反应与异氟醚过量的阳性相关性。所提出的SAR-OCT设计和图像过程技术提供了一种能够检测小鼠视网膜成像的子分辨率神经元变化的对比机制。

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