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首页> 外文会议>Conference on Optical Coherence tomography and coherence domain optical methods in biomedicine XXI >Correlation between polarization sensitive optical coherence tomography and SHG microscopy in articular cartilage
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Correlation between polarization sensitive optical coherence tomography and SHG microscopy in articular cartilage

机译：关节软骨偏振敏感光学相干断层扫描与SHG显微镜的相关性

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Polarization-sensitive optical coherence tomography (PS-OCT) and second harmonic generation (SHG) microscopy are two imaging modalities with different resolutions, field-of-views (FOV), and contrasts, while they both have the capability of imaging collagen fibers in biological tissues. PS-OCT can measure the tissue birefringence which is induced by highly organized fibers while SHG can image the collagen fiber organization with high resolution. Articular cartilage, with abundant structural collagen fibers, is a suitable sample to study the correlation between PS-OCT and SHG microscopy. Qualitative conjecture has been made that the phase retardation measured by PS-OCT is affected by the relationship between the collagen fiber orientation and the illumination direction. Anatomical studies show that the multilayered architecture of articular cartilage can be divided into four zones from its natural surface to the subchondral bone: the superficial zone, the middle zone, the deep zone, and the calcified zone. The different zones have different collagen fiber orientations, which can be studied by the different slopes in the cumulative phase retardation in PS-OCT. An algorithm is developed based on the quantitative analysis of PS-OCT phase retardation images to analyze the microstructural features in swine articular cartilage tissues. This algorithm utilizes the depth-dependent slope changing of phase retardation A-lines to segment structural layers. The results show good consistency with the knowledge of cartilage morphology and correlation with the SHG images measured at selected depth locations. The correlation between PS-OCT and SHG microscopy shows that PS-OCT has the potential to analyze both the macro and micro characteristics of biological tissues with abundant collagen fibers and other materials that may cause birefringence.
机译：偏振敏感光学相干断层扫描（PS-OCT）和二次谐波产生（SHG）显微镜是具有不同分辨率的两个成像模态，场的视图（FOV），和反差，而它们都具有成像胶原纤维中的能力生物组织。 PS-OCT可以测量其通过高度组织的光纤，而SHG罐图像胶原纤维组织具有高分辨率引起的组织的双折射。关节软骨，具有丰富的结构的胶原纤维，是合适的样品，研究PS-OCT和SHG显微镜之间的相关性。定性猜想已经取得，通过PS-OCT测量的相位延迟是由胶原纤维取向和照射方向之间的关系的影响。解剖学研究表明，关节软骨的多层架构可以分成四个区从其天然表面软骨下骨：浅区，中间区，深区和钙化区。不同的区域具有不同的胶原纤维取向，这可以通过在PS-OCT的累积相位延迟的不同斜率进行研究。的算法是基于PS-OCT相位延迟图像的定量分析来分析在猪关节软骨组织中的微结构化特征的发展。该算法利用所述深度相关斜率的相位延迟A线到段结构层发生变化。结果表明与软骨形态学和相关性的知识在选定深度位置处测得的SHG图像很好的一致性。该PS-OCT必须分析宏观和具有丰富的胶原纤维和其他材料，可能会导致双折射的生物组织的微特征的潜力PS-OCT和SHG显微镜显示之间的相关性。

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